Literature summary for 3.1.21.8 extracted from

Lagerbaeck, P.; Carlson, K.
Amino acid residues in the GIY-YIG endonuclease II of phage T4 affecting sequence recognition and binding as well as catalysis (2008), J. Bacteriol., 190, 5533-5544.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Eschgerichia coli	Tequatrovirus T4

Crystallization (Commentary)

Crystallization (Comment)	Organism
modeling based on structure of UvrC, PDB code 1YD0. Residues G49, R57, E118, and N130 in the putative catalytic surface are important for substrate recognition and binding. Residues G49 and R57 are essential for normal sequence recognition. and play a role in forming the DNA-binding surface and exposing the substrate scissile bond at the active site. Residues N130 and P127 likely contribute to positioning the catalytic domain correctly. Residue K76, part of a conserved NUMOD3 DNA-binding motif of homing endonucleases found to overlap the MR, affects both sequence recognition and catalysis	Tequatrovirus T4

Crystallization (Comment)

Organism

modeling based on structure of UvrC, PDB code 1YD0. Residues G49, R57, E118, and N130 in the putative catalytic surface are important for substrate recognition and binding. Residues G49 and R57 are essential for normal sequence recognition. and play a role in forming the DNA-binding surface and exposing the substrate scissile bond at the active site. Residues N130 and P127 likely contribute to positioning the catalytic domain correctly. Residue K76, part of a conserved NUMOD3 DNA-binding motif of homing endonucleases found to overlap the MR, affects both sequence recognition and catalysis

Tequatrovirus T4

Protein Variants

Protein Variants	Comment	Organism
A83L	similar to wild-type, cleavage activity is 20 to 70% of the nicking activity	Tequatrovirus T4
E118A	complete loss of cleavage activity	Tequatrovirus T4
G49A	complete loss of cleavage activity	Tequatrovirus T4
I24A	similar to wild-type, cleavage activity is 20 to 70% of the nicking activity	Tequatrovirus T4
K12A	similar to wild-type, cleavage activity is 20 to 70% of the nicking activity	Tequatrovirus T4
K76A	much reduced catalytic activity	Tequatrovirus T4
L16A	similar to wild-type, cleavage activity is 20 to 70% of the nicking activity	Tequatrovirus T4
L84P	much reduced catalytic activity	Tequatrovirus T4
N130A	complete loss of cleavage activity	Tequatrovirus T4
P127L	much reduced catalytic activity	Tequatrovirus T4
R57A	cleavage activity is 5 to 7% of the nicking activity	Tequatrovirus T4
S72A	similar to wild-type, cleavage activity is 20 to 70% of the nicking activity	Tequatrovirus T4
V90A	similar to wild-type, cleavage activity is 20 to 70% of the nicking activity	Tequatrovirus T4

Metals/Ions

Metals/Ions	Comment	Organism
Mg2+	required	Tequatrovirus T4
Mn2+	compared to Mg2+, relative nicking activity is 0.4 with top strand, 0.8 with bottom strand	Tequatrovirus T4
additional information	ca2+ cannot substitute Mg2+	Tequatrovirus T4
Ni2+	compared to Mg2+, relative nicking activity is 0.1 with top strand, 0.1 with bottom strand	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	P07059	-	-

Synonyms

Synonyms	Comment	Organism
denA	-	Tequatrovirus T4