Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Haemophilus influenzae | |
Mg2+ | required | Escherichia coli | |
Mn2+ | Mg2+ can be replaced by Mn2+ albeit with lower efficiency | Haemophilus influenzae | |
Mn2+ | Mg2+ can be replaced by Mn2+ albeit with lower efficiency | Escherichia coli | |
additional information | not activated by Ca2+ and Na+ | Haemophilus influenzae | |
additional information | not activated by Ca2+ and Na+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, EcoRI, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Escherichia coli | cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
double-stranded DNA + H2O | Escherichia coli | cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
double-stranded DNA + H2O | Haemophilus influenzae | cleavage by HindII is blunt, producing fragments with flush ends | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Haemophilus influenzae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
double-stranded DNA + H2O | cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
double-stranded DNA + H2O | cleavage by HindII is blunt, producing fragments with flush ends | Haemophilus influenzae | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x * 30000, EcoRI, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
EcoRI | - |
Escherichia coli |
EcoRV | - |
Escherichia coli |
HindII | - |
Haemophilus influenzae |
type II REase | - |
Haemophilus influenzae |
type II REase | - |
Escherichia coli |
type II restriction endonuclease | - |
Haemophilus influenzae |
type II restriction endonuclease | - |
Escherichia coli |