Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | required | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE | Escherichia coli |
55000 | - |
alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE | Escherichia coli |
60000 | - |
alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE | Escherichia coli |
62000 | - |
alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE | Escherichia coli |
135000 | - |
alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE | Escherichia coli |
135000 | - |
alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE | Escherichia coli |
400000 | - |
EcoK, sucrose density gradient sedimentation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Salmonella enterica subsp. enterica serovar Typhimurium | enzyme restricts the exchange of genetic material between bacteria of different strains or species | ? | - |
? | |
additional information | Escherichia coli | enzyme restricts the exchange of genetic material between bacteria of different strains or species | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
enzyme EcoR | - |
Escherichia coli | - |
enzyme EcoK | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
enzyme SA and SB | - |
Purification (Comment) | Organism |
---|---|
enzyme EcoB | Escherichia coli |
enzyme EcoK | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
duplex DNA + ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate | - |
? | |
duplex DNA + ATP | the site of restriction cleavage is random, occuring between 1 and 5 kb from the recognition site. The modification methylase acts directly at the recognition sequence | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate | - |
? | |
additional information | enzyme restricts the exchange of genetic material between bacteria of different strains or species | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
additional information | enzyme restricts the exchange of genetic material between bacteria of different strains or species | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE | Escherichia coli |
pentamer | alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
EcoK | - |
Escherichia coli |
EcoR | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
167 | - |
ATP | at 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
nuclease reaction | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | required | Escherichia coli | |
ATP | is degraded during reaction | Escherichia coli |