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Literature summary for 3.1.21.10 extracted from
- Activation of RuvC Holliday junction resolvase in vitro (1994), Nucleic Acids Res., 22, 2490-2497 .
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in assay conditions | Escherichia coli |  |
| Mn2+ | Mn2+ substitutes as an efficient cofactor for cleavage by the enzyme, but does not stimulate resolution activity | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A814 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| duplex DNA | - |
Escherichia coli | ? | - |
? | |
| synthetic Holliday junction X10 | synthetic DNA junction, containing the original 12 base pair core of homology, in which the region of homology is extended to a length of 10 base pairs | Escherichia coli | ? | - |
? | |
| synthetic Holliday junction X12 | synthetic DNA junction, containing the original 12 base pair core of homology, in which the region of homology is extended to a length of 12 base pairs | Escherichia coli | ? | - |
? | |
| synthetic Holliday junction X26 | synthetic DNA junction, containing the original 12 base pair core of homology, in which the region of homology is extended to a length of 26 base pairs | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Holliday junction resolvase | - |
Escherichia coli |
| RuvC | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 65 | the optimum temperature for resolution is 65°C, with 70% of the substrate resolved within 15 min. At 37°C, less than 5% resolution is observed after 15 min, rising to a maximum level of resolution of 35% after 3 h | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
in the presence of Mn2+, resolution activity is optimal at physiological pH (about 8.0) | Escherichia coli |
| 10 | - |
in Mg2+-dependent reactions, resolution is greatest at pH 10.0 | Escherichia coli |
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