Literature summary for 3.1.21.10 extracted from

Zecchi, L.; Lo Piano, A.; Suzuki, Y.; Canas, C.; Takeyasu, K.; Ayora, S.
Characterization of the Holliday junction resolving enzyme encoded by the Bacillus subtilis bacteriophage SPP1 (2012), PLoS ONE, 7, e48440.

Search for more literature for 3.1.21.10

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells	Bacillus phage SPP1

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity	Bacillus phage SPP1

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27500	-	2 * 27500, calculated from amino acid sequence	Bacillus phage SPP1
32000	-	2 * 32000, SDS-PAGE	Bacillus phage SPP1
54000	-	gel filtration	Bacillus phage SPP1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA + H2O	Bacillus phage SPP1	the enzyme from gene G44P preferentially cleaves Holliday junctions, but also, with lower efficiency, replicated D-loops	hydrolyzed DNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus phage SPP1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
hydroxyapatite column chromatography, MonoQ column chromatography, and Superdex 75 gel filtration	Bacillus phage SPP1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	the enzyme from gene G44P preferentially cleaves Holliday junctions, but also, with lower efficiency, replicated D-loops	Bacillus phage SPP1	hydrolyzed DNA	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 32000, SDS-PAGE	Bacillus phage SPP1
homodimer	2 * 27500, calculated from amino acid sequence	Bacillus phage SPP1

Synonyms

Synonyms	Comment	Organism
G44P protein	-	Bacillus phage SPP1
Holliday junction resolving enzyme	-	Bacillus phage SPP1

General Information

General Information	Comment	Organism
malfunction	deletion of the enzyme leads to delay in DNA synthesis	Bacillus phage SPP1
physiological function	the enzyme plays a role in replication restart during the transition to concatemeric viral replication	Bacillus phage SPP1

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Release 2023.1 (January 2023)