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Literature summary for 3.1.21.10 extracted from

  • Suzuki, Y.; Endo, M.; Canas, C.; Ayora, S.; Alonso, J.C.; Sugiyama, H.; Takeyasu, K.
    Direct analysis of Holliday junction resolving enzyme in a DNA origami nanostructure (2014), Nucleic Acids Res., 42, 7421-7428.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) [pLysS] cells Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme requires high concentrations of Mg2+ (10-15 mM) for activity Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Bacillus subtilis
-
hydrolyzed DNA
-
?
DNA + H2O Bacillus subtilis 168
-
hydrolyzed DNA
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P39792
-
-
Bacillus subtilis 168 P39792
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Bacillus subtilis hydrolyzed DNA
-
?
DNA + H2O
-
Bacillus subtilis 168 hydrolyzed DNA
-
?

Synonyms

Synonyms Comment Organism
Holliday junction resolving enzyme
-
Bacillus subtilis
RecU
-
Bacillus subtilis