Literature summary for 3.1.21.10 extracted from

Sukackaite, R.; Lagunavicius, A.; Stankevicius, K.; Urbanke, C.; Venclovas, C.; Siksnys, V.
Restriction endonuclease BpuJI specific for the 5-CCCGT sequence is related to the archaeal Holliday junction resolvase family (2007), Nucleic Acids Res., 35, 2377-2389.

Search for more literature for 3.1.21.10

Cloned(Commentary)

Cloned (Comment)	Organism
for expression in Escherichia coli ER2267 cells	Bacillus pumilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	BpuJI requires Mg2+-ions for DNA cleavage	Bacillus pumilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53900	-	predicted molecular mass of the BpuJI subunit	Bacillus pumilus
109000	-	homodimer, determined by sedimentation equilibrium experiments using an analytical ultracentrifuge	Bacillus pumilus

Organism

Organism	UniProt	Comment	Textmining
Bacillus pumilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using heparin-Sepharose, blue-Sepharose and AH-Sepharose columns	Bacillus pumilus

Subunits

Subunits	Comment	Organism
homodimer	-	Bacillus pumilus

Synonyms

Synonyms	Comment	Organism
BpuJI	BpuJI is cleaved into a N-terminal domain, NTD, binding domain, and a C-terminal domain, CTD, catalytic domain, CTD is structurally related to archaeal Holliday junction resolvases	Bacillus pumilus
Holliday junction resolvase	-	Bacillus pumilus
REase	-	Bacillus pumilus
restriction endonuclease	-	Bacillus pumilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	PCR fragment cleavage assay	Bacillus pumilus
25	-	plasmid and lambda DNA cleavage assay	Bacillus pumilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	PCR fragment cleavage assay	Bacillus pumilus
7.5	-	plasmid and lambda DNA cleavage assay	Bacillus pumilus

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Release 2023.1 (January 2023)