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Literature summary for 3.1.21.10 extracted from

  • White, M.F.; Lilley, D.M.J.
    Characterizaton of a holliday junction-resolving enzyme from Schizosaccharomyces pombe (1997), Mol. Cell. Biol., 17, 6465-6471.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.10

Cloned(Commentary)

Cloned (Comment) Organism
construction of a MBP-YDC2 fusion protein Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
-
 Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Schizosaccharomyces pombe holliday structure hydrolyzed DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant fusion protein MBP-YDC2 Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O cleaves at the recognition sequence 5'-CT, preference for the point of strand exchange of fixed junctions Schizosaccharomyces pombe hydrolyzed DNA
-
 ?
DNA + H2O holliday structure Schizosaccharomyces pombe hydrolyzed DNA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000, binds to the holliday junction as a dimer Schizosaccharomyces pombe
dimer exists as a monomer-dimer equilibrium in solution Schizosaccharomyces pombe

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 for 2 min followed by transfer on ice, inactivation Schizosaccharomyces pombe