Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.2.6 extracted from

  • Uotila, L.
    Purification and characterization of S-2-hydroxyacylglutathione hydrolase (glyoxalase II) from human liver (1973), Biochemistry, 12, 3944-3951.
    View publication on PubMed
Search for more literature for 3.1.2.6

General Stability

General Stability Organism
50% loss of activity on freezing and thawing Homo sapiens
bovine serum albumin and glycerol, 30% v/v remove the sensitivity to freezing Homo sapiens
dialysis, 50% loss of activity after 8 h Homo sapiens
EDTA, 1 mM, labilizes the enzyme in long-term storage Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzenesulfonate
-
 Homo sapiens
AsO2-
-
 Homo sapiens
CdCl2
-
 Homo sapiens
copper acetate above 15 mM Homo sapiens
diphosphate 25 mM, 50% inhibition Homo sapiens
glutathione weak competitive Homo sapiens
Hg2+ HgCl2, most potent inhibitor Homo sapiens
KCl above 15 mM Homo sapiens
NaCl above 15 mM Homo sapiens
NH4Cl above 15 mM Homo sapiens
oxalate 20 mM, 38% inhibition Homo sapiens
phosphate 67 mM, 35% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0164
-
 S-mandeloylglutathione
-
 Homo sapiens
0.07
-
 S-glycolylglutathione
-
 Homo sapiens
0.109
-
 S-glyceroylglutathione
-
 Homo sapiens
0.153
-
 S-formylglutathione
-
 Homo sapiens
0.19
-
 S-Lactoylglutathione
-
 Homo sapiens
0.2
-
 S-succinylglutathione
-
 Homo sapiens
0.213
-
 S-propionylglutathione
-
 Homo sapiens
0.266
-
 S-acetylglutathione
-
 Homo sapiens
0.296
-
 S-Acetoacetylglutathione
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22900
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
822
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactoylglutathione + H2O
-
 Homo sapiens D-lactate + glutathione
-
 ?
S-acetoacetylglutathione + H2O 55.6% of the activity with S-lactoylglutathione Homo sapiens acetoacetate + glutathione
-
 ?
S-acetylglutathione + H2O 8.6% of the activity with S-lactoylglutathione Homo sapiens acetate + glutathione
-
 ?
S-formylglutathione + H2O 37.7% of the activity with S-lactoylglutathione Homo sapiens formate + glutathione
-
 ?
S-glyceroylglutathione + H2O
-
 Homo sapiens glycerate + glutathione
-
 ?
S-glycerylglutathione + H2O 61.6% of the activity with S-lactoylglutathione Homo sapiens glycerate + glutathione
-
 ?
S-glycoloylglutathione + H2O 38.6% of the activity with S-lactoylglutathione Homo sapiens glycolate + glutathione
-
 ?
S-lactoylglutathione + H2O
-
 Homo sapiens D-lactate + glutathione
-
 ?
S-mandeloylglutathione + H2O 4.8% of the activity with S-lactoylglutathione Homo sapiens mandelate + glutathione
-
 ?
S-propionylglutathione + H2O 13.5% of the activity with S-lactoylglutathione Homo sapiens propionate + glutathione
-
 ?
S-succinylglutathione + H2O 29.2% of the activity with S-lactoylglutathione Homo sapiens succinate + glutathione
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 pH 7, 5 min, stable up to Homo sapiens
45
-
 pH 7, 5 min, 50% loss of activity Homo sapiens
60
-
 pH 7, 5 min, complete inactivation Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.5
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
25
-
 diphosphate
-
 Homo sapiens