Crystallization (Comment) | Organism |
---|---|
structure of recombinant PqsE. The Protein possesses a metallo-beta-lactamase fold with an Fe(II)Fe(III) center in the active site. A copurified ligand is benzoate | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E182A | significant decrease in kcat value for S-(4-nitrobenzoyl)mercaptoethane | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
S-(4-nitrobenzoyl)mercaptoethane | mutant E182A, pH 8.5, 25°C, presence of Mn2+ | Pseudomonas aeruginosa | |
0.014 | - |
S-(4-nitrobenzoyl)mercaptoethane | wild-type, pH 8.5, 25°C, presence of Mn2+ | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 9.7fold stimulation of EDTA-treated protein | Pseudomonas aeruginosa | |
Fe2+ | 3.3fold stimulation of EDTA-treated protein. Protein has a Fe(II)Fe(III) center in the active site, crystallization data | Pseudomonas aeruginosa | |
Mn2+ | 7.9fold stimulation of EDTA-treated protein | Pseudomonas aeruginosa | |
Ni2+ | 1.9fold stimulation of EDTA-treated protein | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P20581 | - |
- |
Pseudomonas aeruginosa DSM 22644 | P20581 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoylacetyl-CoA + H2O | - |
Pseudomonas aeruginosa | 2-aminobenzoylacetate + CoA | - |
? | |
2-aminobenzoylacetyl-CoA + H2O | - |
Pseudomonas aeruginosa DSM 22644 | 2-aminobenzoylacetate + CoA | - |
? | |
additional information | the enzyme slowly hydrolyzes phosphodiesters including single- and double-strandedDNA as well as mRNA and also the thioester S-(4-nitrobenzoyl)mercaptoethane. No substrates: acetyl-CoA, malonyl-CoA, benzoyl-CoA, L-lactoylglutathione | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme slowly hydrolyzes phosphodiesters including single- and double-strandedDNA as well as mRNA and also the thioester S-(4-nitrobenzoyl)mercaptoethane. No substrates: acetyl-CoA, malonyl-CoA, benzoyl-CoA, L-lactoylglutathione | Pseudomonas aeruginosa DSM 22644 | ? | - |
? | |
S-(4-nitrobenzoyl)mercaptoethane + H2O | - |
Pseudomonas aeruginosa | 4-nitrobenzoate + mercaptoethane | - |
? | |
S-(4-nitrobenzoyl)mercaptoethane + H2O | - |
Pseudomonas aeruginosa DSM 22644 | 4-nitrobenzoate + mercaptoethane | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PA1000 | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
S-(4-nitrobenzoyl)mercaptoethane | mutant E182A, pH 8.5, 25°C, presence of Mn2+ | Pseudomonas aeruginosa | |
0.12 | - |
S-(4-nitrobenzoyl)mercaptoethane | wild-type, pH 8.5, 25°C, presence of Mn2+ | Pseudomonas aeruginosa |