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Literature summary for 3.1.2.14 extracted from

  • Ziesack, M.; Rollins, N.; Shah, A.; Dusel, B.; Webster, G.; Silver, P.A.; Way, J.C.
    Chimeric fatty acyl-acyl carrier protein thioesterases provide mechanistic insight into enzyme specificity and expression (2018), Appl. Environ. Microbiol., 84, e02868-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.2.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cuphea palustris

Protein Variants

Protein Variants Comment Organism
E235Q the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
L242F the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
M237V the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
S225N the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
S249G the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
S269A the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an oleoyl-[acyl-carrier protein] + H2O Cuphea palustris
-
 an [acyl-carrier protein] + oleate
-
 ?

Organism

Organism UniProt Comment Textmining
Cuphea palustris Q39554 and Q39555 Q39554 for isoform FatB1 and Q39555 for isoform FatB2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an oleoyl-[acyl-carrier protein] + H2O
-
 Cuphea palustris an [acyl-carrier protein] + oleate
-
 ?
myristoyl-[acyl-carrier protein] + H2O high specificity substrate of isoform FatB2 Cuphea palustris [acyl-carrier protein] + myristate
-
 ?
octanoyl-[acyl-carrier protein] + H2O high specificity substrate of isoform FatB1 Cuphea palustris [acyl-carrier protein] + octanoate
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Cuphea palustris

Synonyms

Synonyms Comment Organism
FATB1 isoform, specific for C8 Cuphea palustris
FatB2 isoform, specific for C14 Cuphea palustris