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Literature summary for 3.1.2.14 extracted from

  • Feng, Y.; Wang, Y.; Liu, J.; Liu, Y.; Cao, X.; Xue, S.
    Structural insight into acyl-ACP thioesterase toward substrate specificity design (2017), ACS Chem. Biol., 12, 2830-2836 .
    View publication on PubMed
Search for more literature for 3.1.2.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Umbellularia californica

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M (NH4)2SO4, 0.1 M Tris-HCl pH 8.5, 20% (w/v) PEG3350 Umbellularia californica

Protein Variants

Protein Variants Comment Organism
F201W the mutant shows 14.1% specific activity compared to the wild type enzyme Umbellularia californica
M163W almost inactive Umbellularia californica
M197R the mutant shows 39.8% specific activity compared to the wild type enzyme Umbellularia californica
N283A almost inactive Umbellularia californica
R199H the mutant shows 0.4% specific activity compared to the wild type enzyme Umbellularia californica
S219W almost inactive Umbellularia californica
T137G the mutant demonstrates comparative relative activity on C14 substrates compared to C12 substrates in vitro and achieves C14 fatty acid content up to 40% in contrast to 10% C14 from the wild type Umbellularia californica
T137Y inactive Umbellularia californica

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Umbellularia californica 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lauroyl-[acyl-carrier protein] + H2O Umbellularia californica
-
 [acyl-carrier protein] + laurate
-
 ?

Organism

Organism UniProt Comment Textmining
Umbellularia californica Q41635
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Umbellularia californica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caproyl-[acyl-carrier protein] + H2O very low activity Umbellularia californica [acyl-carrier protein] + caproate
-
 ?
lauroyl-[acyl-carrier protein] + H2O
-
 Umbellularia californica [acyl-carrier protein] + laurate
-
 ?
lauroyl-[acyl-carrier protein] + H2O high activity Umbellularia californica [acyl-carrier protein] + laurate
-
 ?
myristoyl-[acyl-carrier protein] + H2O very low activity Umbellularia californica [acyl-carrier protein] + myristate
-
 ?
palmitoyl-[acyl-carrier protein] + H2O very low activity Umbellularia californica [acyl-carrier protein] + palmitate
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Umbellularia californica

Synonyms

Synonyms Comment Organism
acyl-ACP-thioesterase
-
 Umbellularia californica
C12-specific thioesterase
-
 Umbellularia californica
FatB
-
 Umbellularia californica