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Literature summary for 3.1.16.1 extracted from

  • Burkovics, P.; Szukacsov, V.; Unk, I.; Haracska, L.
    Human Ape2 protein has a 3-5 exonuclease activity that acts preferentially on mismatched base pairs (2006), Nucleic Acids Res., 34, 2508-2515.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.16.1

Application

Application Comment Organism
additional information Ape2 exhibits strong 3'-5' exonuclease and 3'-phosphodiesterase activities and has only a very weak AP-endonuclease activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
full-length, sequence verified PCR product cloned into vector pENTR3C, wild type and mutant Ape2 cDNAs in fusion with GST under the control of the Saccharomyces cerevisiae galactose-inducible phosphoglycerate promoter using plasmid pBJ842, resulting in plasmids pIL1047 and pIL1247, respectively, overexpressed in parallel in the protease-deficient BJ5464 yeast strain Homo sapiens

Protein Variants

Protein Variants Comment Organism
D277A mutation of the active-site residue, inactivates 3'–5' exonuclease, 3'-phosphodiesterase and AP-endonuclease activities Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NaCl at 100 mM Ape2 exhibits only 20% of its exonuclease activity, in the presence of 200 mM salt almost no exonuclease activity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens
Mn2+ 5fold more active in the presence of manganese than of magnesium ion Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Homo sapiens

Storage Stability

Storage Stability Organism
-80°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O Ape2 preferentially removes mismatched 3'-nucleotides from DNA Homo sapiens deoxyribonucleoside 3'-phosphates
-
 ?

Synonyms

Synonyms Comment Organism
3'-5' exonuclease
-
 Homo sapiens
Ape2
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Homo sapiens