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Literature summary for 3.1.14.1 extracted from

  • Shetty, J.K.; Weaver, R.C.; Kinsella, J.E.
    A rapid method for the isolation of ribonuclease from yeast (Saccharomyces carlsbergensis) (1980), Biochem. J., 189, 363-366.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.14.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM: inhibition of intracellular proteolytic proteins during the incubation of disrupted yeast cells for RNA hydrolysis Saccharomyces pastorianus
phenylmethanesulfonyl fluoride 0.1 mM: inhibition of intracellular proteolytic proteins during the incubation of disrupted yeast cells for RNA hydrolysis Saccharomyces pastorianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleic acid + H2O Saccharomyces pastorianus exonucleolytic cleavage 3'-phosphomononucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces pastorianus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces pastorianus

Reaction

Reaction Comment Organism Reaction ID
exonucleolytic cleavage to nucleoside 3'-phosphates exonucleolytic cleavage Saccharomyces pastorianus
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information released from disrupted cells Saccharomyces pastorianus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces pastorianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides
-
 ?
ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides no base specificity ?
ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces pastorianus 3'-phosphomononucleotides 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?

Synonyms

Synonyms Comment Organism
RNase
-
 Saccharomyces pastorianus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
 Saccharomyces pastorianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Saccharomyces pastorianus