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Literature summary for 3.1.13.5 extracted from

  • Ghosh, R.K.; Deutscher, M.P.
    Identification of an Escherichia coli nuclease acting on structurally altered transfer RNA molecules (1978), J. Biol. Chem., 253, 997-1000.
    View publication on PubMed
Search for more literature for 3.1.13.5

Activating Compound

Activating Compound Comment Organism Structure
NH4+ stimulates Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibition can be reversed by Mg2+ Escherichia coli
Na+ slight Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates Escherichia coli
Mg2+ required, optimal concentration is 5 mM Escherichia coli
Mn2+ can partially replace for Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the enzyme may represent an intracellular scavenging mechanism for denatured tRNAs and other inactive RNA molecules ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme may represent an intracellular scavenging mechanism for denatured tRNAs and other inactive RNA molecules Escherichia coli ?
-
 ?
structurally altered tRNA exonuclease activity. RNase D can recognize structurally altered tRNA molecules. The enzyme acts poorly on intact tRNA and is inactive with the synthetic polyribonucleotides, poly(A), poly(U), or double-stranded poly(A)*poly(U). The enzyme acts on diesterase-treated tRNA, but relatively poorly on intact tRNA. RNase D does not attack ribosomal RNA Escherichia coli 5'-mononucleotide + ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9 with diesterase-treated tRNA as substrate Escherichia coli