Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta DE3 cells | Human spumaretrovirus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | two Mg2+ ions are bound in the catalytic center. The purified RNase H domain is stable in the presence of Mg2+ ions | Human spumaretrovirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
1 * 18000, calculated from amino acid sequence | Human spumaretrovirus |
19000 | - |
gel filtration | Human spumaretrovirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | Human spumaretrovirus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human spumaretrovirus | - |
foamy virus | - |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography and gel filtration | Human spumaretrovirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | - |
Human spumaretrovirus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 18000, calculated from amino acid sequence | Human spumaretrovirus |
Synonyms | Comment | Organism |
---|---|---|
RNase H | - |
Human spumaretrovirus |