Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | human BLM helicase, a member of the RecQ family, stimulates the nucleolytic activity of Exo1. Stimulation of hExo1 by BLM does not require ATP. Stimulation is specific because other RecQ homologs (human WRN, RecQ1, RecQ4 and RecQ5beta and Escherichia coli RecQ) fail to stimulate Exo1. RPA does not affect the nuclease activity of Exo1 alone, nor does it appreciably change substrate utilization by Exo1 in the presence of BLM helicase. BLM helicase physically interacts with Exo1 | Homo sapiens |
Application | Comment | Organism |
---|---|---|
additional information | role of BLM and Exo1 in the initiation of recombinational DNA repair. Stimulation of DNA resection by human Exo1 is independent of BLM helicase activity and is, instead, mediated by an interaction between the two proteins. DNA ends resected by Exo1 and BLM are used by human Rad51, but not its yeast or bacterial counterparts, to promote homologous DNA pairing | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D173A | with either mutant D173A alone or both mutant D173A and BLM, nucleolytic degradation is undetectable. It competitively inhibits Exo1-BLM-mediated resection | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Escherichia coli RecQ inhibits Exo1, most likely by blocking Exo1 access to the ends of DNA | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-end-labeled dsDNA + H2O | - |
Homo sapiens | ? | - |
? | |
5'-end-labeled dsDNA + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Exo1 | - |
Homo sapiens |
exonuclease 1 | - |
Homo sapiens |