Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Hydrogenophaga intermedia |
expressed in Escherichia coli | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
4-sulfomuconolactone | pH 8.0, temperature not specified in the publication | Agrobacterium tumefaciens | |
1.9 | - |
4-sulfomuconolactone | pH 8.0, temperature not specified in the publication | Hydrogenophaga intermedia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 0.6 mol of Zn2+ per mol of enzyme | Hydrogenophaga intermedia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | A6XIG7 | - |
- |
Agrobacterium tumefaciens DSM Z 5681 | A6XIG7 | - |
- |
Hydrogenophaga intermedia | A6XIG6 | - |
- |
Hydrogenophaga intermedia DSM Z 5680 | A6XIG6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Hydrogenophaga intermedia |
- |
Agrobacterium tumefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.6 | - |
pH 8.0, temperature not specified in the publication | Agrobacterium tumefaciens |
79 | - |
pH 8.0, temperature not specified in the publication | Hydrogenophaga intermedia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-sulfomuconolactone + H2O | - |
Hydrogenophaga intermedia | maleylacetate + sulfite | - |
? | |
4-sulfomuconolactone + H2O | - |
Agrobacterium tumefaciens | maleylacetate + sulfite | - |
? | |
4-sulfomuconolactone + H2O | - |
Hydrogenophaga intermedia DSM Z 5680 | maleylacetate + sulfite | - |
? | |
4-sulfomuconolactone + H2O | - |
Agrobacterium tumefaciens DSM Z 5681 | maleylacetate + sulfite | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
4-sulfomuconolactone | pH 8.0, temperature not specified in the publication | Agrobacterium tumefaciens | |
45 | - |
4-sulfomuconolactone | pH 8.0, temperature not specified in the publication | Hydrogenophaga intermedia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
4-sulfomuconolactone | pH 8.0, temperature not specified in the publication | Agrobacterium tumefaciens | |
23 | - |
4-sulfomuconolactone | pH 8.0, temperature not specified in the publication | Hydrogenophaga intermedia |