Literature summary for 3.1.1.92 extracted from

  • Halak, S.; Basta, T.; Bürger, S.; Contzen, M.; Wray, V.; Pieper, D.H.;, Stolz, A.
    4-sulfomuconolactone hydrolases from Hydrogenophaga intermedia S1 and Agrobacterium radiobacter S2 (2007), J. Bacteriol., 189, 6998-7006.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Hydrogenophaga intermedia
expressed in Escherichia coli Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
4-sulfomuconolactone pH 8.0, temperature not specified in the publication Hydrogenophaga intermedia
0.34
-
4-sulfomuconolactone pH 8.0, temperature not specified in the publication Agrobacterium tumefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 0.6 mol of Zn2+ per mol of enzyme Hydrogenophaga intermedia

Organism

Organism UniProt Comment Textmining
Hydrogenophaga intermedia A6XIG6
-
-
Agrobacterium tumefaciens A6XIG7
-
-
Hydrogenophaga intermedia DSM Z 5680 A6XIG6
-
-
Agrobacterium tumefaciens DSM Z 5681 A6XIG7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hydrogenophaga intermedia
-
Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
79
-
pH 8.0, temperature not specified in the publication Hydrogenophaga intermedia
12.6
-
pH 8.0, temperature not specified in the publication Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-sulfomuconolactone + H2O
-
Hydrogenophaga intermedia maleylacetate + sulfite
-
?
4-sulfomuconolactone + H2O
-
Agrobacterium tumefaciens maleylacetate + sulfite
-
?
4-sulfomuconolactone + H2O
-
Hydrogenophaga intermedia DSM Z 5680 maleylacetate + sulfite
-
?
4-sulfomuconolactone + H2O
-
Agrobacterium tumefaciens DSM Z 5681 maleylacetate + sulfite
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
45
-
4-sulfomuconolactone pH 8.0, temperature not specified in the publication Hydrogenophaga intermedia
6.7
-
4-sulfomuconolactone pH 8.0, temperature not specified in the publication Agrobacterium tumefaciens

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
20
-
4-sulfomuconolactone pH 8.0, temperature not specified in the publication Agrobacterium tumefaciens
23
-
4-sulfomuconolactone pH 8.0, temperature not specified in the publication Hydrogenophaga intermedia