Application | Comment | Organism |
---|---|---|
environmental protection | the mutant enzyme A171V/D256N is an ideal candidate for the biodegradation of pyrethroids (widely used as insecticides) | uncultured bacterium |
Cloned (Comment) | Organism |
---|---|
extracellularly expressed in a Pichia pastoris host system | uncultured bacterium |
Protein Variants | Comment | Organism |
---|---|---|
A171V | the mutant displays 2.46fold higher catalytic activity with the substrate 4-nitrophenyl acetate | uncultured bacterium |
A171V/D256N | the mutant displays 2.46fold higher catalytic activity with the substrate 4-nitrophenyl acetate. The mutant is able to efficiently degrade all the pyrethroids (cyhalothrin, cypermethrin, sumicidin, deltamethrin) tested within a short time, indicating that the mutant possesses broad substrate specificity. It shows a broad substrate specificity. The optimal temperature is 10°C higher than that of the wild-type enzyme (55°C). The half-life at 40-65°C is 3.3-310 times longer. The mutant enzyme has a half-life of 12 h at 70°C while the wild-type Sys410 is completely inactivated at 70°C | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01161 | - |
4-nitrophenyl acetate | mutant enzyme A171V/D256N, pH 6.5, 55°C | uncultured bacterium | |
0.0137 | - |
4-nitrophenyl acetate | mutant enzyme A171V, pH 6.5, 55°C | uncultured bacterium | |
0.1411 | - |
4-nitrophenyl acetate | wild-type enzyme, pH 6.5, 55°C | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | H9CDG2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | best substrate for wild-type end mutant enzymes A171V and A171V/D256N | uncultured bacterium | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
uncultured bacterium | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | low activity, wild-type and mutant enzymes A171V and A171V/D256N | uncultured bacterium | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl dodecanoate + H2O | low activity, wild-type and mutant enzymes A171V and A171V/D256N | uncultured bacterium | 4-nitrophenol + dodecanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
uncultured bacterium | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | low activity, wild-type and mutant enzymes A171V and A171V/D256N | uncultured bacterium | 4-nitrophenol + octanoate | - |
? | |
cyhalothrin + H2O | - |
uncultured bacterium | 3-phenoxybenzaldehyde + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
cypermethrin + H2O | - |
uncultured bacterium | 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
deltamethrin + H2O | - |
uncultured bacterium | 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
sumicidin + H2O | - |
uncultured bacterium | 3-phenoxybenzaldehyde + 2-(4-chlorophenyl)-3-methylbutanoic acid + cyanide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
esterase sys410 | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
wild-type enzyme | uncultured bacterium |
65 | - |
mutant enzymes A171V and A171V/D256N | uncultured bacterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 65 | 35°C: about 75% of maximal activity, 65°C: about 65% of maximal activity, wild-type enzyme | uncultured bacterium |
40 | 80 | 40°C: about 55% of maximal activity, 80°C: about 60% of maximal activity, mutant enzyme A171V/D256N | uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
T1/2: 72 h (wild-type enzyme Sys410), 236 h (mutant enzyme A171V/D256N) | uncultured bacterium |
45 | - |
T1/2: 38 h (wild-type enzyme Sys410), 154 h (mutant enzyme A171V/D256N) | uncultured bacterium |
50 | - |
T1/2: 10 h (wild-type enzyme Sys410), 133 h (mutant enzyme A171V/D256N) | uncultured bacterium |
55 | - |
T1/2: 6 h (wild-type enzyme Sys410), 77 h (mutant enzyme A171V/D256N) | uncultured bacterium |
60 | - |
T1/2: 84 min (wild-type enzyme Sys410), 53 h (mutant enzyme A171V/D256N) | uncultured bacterium |
65 | - |
T1/2: 6 min (wild-type enzyme Sys410), 31 h (mutant enzyme A171V/D256N) | uncultured bacterium |
70 | - |
T1/2: 12 h (mutant enzyme A171V/D256N) | uncultured bacterium |
80 | - |
T1/2: 4 h (mutant enzyme A171V/D256N) | uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
289.12 | - |
4-nitrophenyl acetate | wild-type enzyme, pH 6.5, 55°C | uncultured bacterium | |
293.31 | - |
4-nitrophenyl acetate | mutant enzyme A171V, pH 6.5, 55°C | uncultured bacterium | |
350.15 | - |
4-nitrophenyl acetate | mutant enzyme A171V/D256N, pH 6.5, 55°C | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | wild-type enzyme | uncultured bacterium |
7 | - |
mutant enzymes A171V and A171V/D256N | uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | pH 4.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity, mutant enzyme A171V/D256N | uncultured bacterium |
4.5 | 8.5 | pH 4.5: about 65% of maximal activity, pH 8.5: about 60% of maximal activity, wild-type enzyme | uncultured bacterium |