Application | Comment | Organism |
---|---|---|
medicine | the wild type cocaine esterase cannot be used as a pharmacotherapy for cocaine abuse because of its 13.7-min half-life at 37°C | Rhodococcus sp. |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme L169K/G173Q, hanging drop vapor diffusion method, using 1.7 M ammonium sulfate, 10 mM Tris-HCl, pH 7.3, and 25 mM NaCl | Rhodococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
L169K/G173Q | highly thermostable mutant with a half-life of 2.9 days at 37°C | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0072 | - |
cocaine | wild type enzyme, without preincubation, at pH 7.4 and 37°C | Rhodococcus sp. | |
0.0073 | - |
cocaine | wild type enzyme, after 24 h preincubation at 37°C, at pH 7.4 and 37°C | Rhodococcus sp. | |
0.0229 | - |
cocaine | mutant enzyme L169K/G173Q, after 24 h preincubation at 37°C, at pH 7.4 and 37°C | Rhodococcus sp. | |
0.0303 | - |
cocaine | mutant enzyme L169K/G173Q, without preincubation, at pH 7.4 and 37°C | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | Q9L9D7 | - |
- |
Rhodococcus sp. MB1 | Q9L9D7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cocaine + H2O | - |
Rhodococcus sp. | ecgonine methyl ester + benzoate | - |
? | |
cocaine + H2O | - |
Rhodococcus sp. MB1 | ecgonine methyl ester + benzoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cocE | - |
Rhodococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the wild type enzyme shows 10.8% residual activity after 24 h at 37°C, mutant enzyme L169K/G173Q shows 77.5% residual activity after 24 h at 37°C | Rhodococcus sp. |