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Literature summary for 3.1.1.84 extracted from
- Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1 (2000), Appl. Environ. Microbiol., 66, 904-908.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the cocE coding sequence was subcloned into the pCFX1 expression plasmid and expressed in Escherichia coli. sequence comparison suggests that cocE encodes a serine esterase | Rhodococcus sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.33 | - |
cocaine | pH 7.5, 30°C | Rhodococcus sp. |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62128 | - |
1 * 62128, calculated from sequence | Rhodococcus sp. |
| 65000 | - |
gel filtration | Rhodococcus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cocaine + H2O | Rhodococcus sp. | Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth. The organism lives in the rhizosphere soil of the tropane alkaloid-producing plant Erythroxylum coca. The cocaine esterase initiates degradation of cocaine, which is hydrolyzed to ecgonine methyl ester and benzoate. Both of these esterolytic products are further metabolized by Rhodococcus sp. strain MB1. This activity is inducible in Rhodococcus sp. strain MB1, since no cocaine esterase activity is observed in cells grown on 15 mM succinate as a sole source of carbon | ecgonine methyl ester + benzoate | - |
? | |
| cocaine + H2O | Rhodococcus sp. MB1 | Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth. The organism lives in the rhizosphere soil of the tropane alkaloid-producing plant Erythroxylum coca. The cocaine esterase initiates degradation of cocaine, which is hydrolyzed to ecgonine methyl ester and benzoate. Both of these esterolytic products are further metabolized by Rhodococcus sp. strain MB1. This activity is inducible in Rhodococcus sp. strain MB1, since no cocaine esterase activity is observed in cells grown on 15 mM succinate as a sole source of carbon | ecgonine methyl ester + benzoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus sp. | Q9L9D7 | - |
- |
| Rhodococcus sp. MB1 | Q9L9D7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Rhodococcus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:cocaine-grown cell | Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth | Rhodococcus sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 43.92 | - |
- |
Rhodococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cocaine + H2O | - |
Rhodococcus sp. | ecgonine methyl ester + benzoate | - |
? | |
| cocaine + H2O | Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth. The organism lives in the rhizosphere soil of the tropane alkaloid-producing plant Erythroxylum coca. The cocaine esterase initiates degradation of cocaine, which is hydrolyzed to ecgonine methyl ester and benzoate. Both of these esterolytic products are further metabolized by Rhodococcus sp. strain MB1. This activity is inducible in Rhodococcus sp. strain MB1, since no cocaine esterase activity is observed in cells grown on 15 mM succinate as a sole source of carbon | Rhodococcus sp. | ecgonine methyl ester + benzoate | - |
? | |
| cocaine + H2O | - |
Rhodococcus sp. MB1 | ecgonine methyl ester + benzoate | - |
? | |
| cocaine + H2O | Rhodococcus sp. MB1 is capable of utilizing cocaine as a sole source of carbon and nitrogen for growth. The organism lives in the rhizosphere soil of the tropane alkaloid-producing plant Erythroxylum coca. The cocaine esterase initiates degradation of cocaine, which is hydrolyzed to ecgonine methyl ester and benzoate. Both of these esterolytic products are further metabolized by Rhodococcus sp. strain MB1. This activity is inducible in Rhodococcus sp. strain MB1, since no cocaine esterase activity is observed in cells grown on 15 mM succinate as a sole source of carbon | Rhodococcus sp. MB1 | ecgonine methyl ester + benzoate | - |
? | |
| additional information | the cocaine esterase displays low levels of activity with 20 mM atropine, a structurally related tropane alkaloid | Rhodococcus sp. | ? | - |
? | |
| additional information | the cocaine esterase displays low levels of activity with 20 mM atropine, a structurally related tropane alkaloid | Rhodococcus sp. MB1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62128, calculated from sequence | Rhodococcus sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cocE | - |
Rhodococcus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rhodococcus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rhodococcus sp. |
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