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Literature summary for 3.1.1.81 extracted from

  • Sirotkina, M.; Efremenko, E.N.
    Rhodococcus lactonase with organophosphate hydrolase (OPH) activity and His6-tagged OPH with lactonase activity: evolutionary proximity of the enzymes and new possibilities in their application (2014), Appl. Microbiol. Biotechnol., 98, 2647-2656.
    View publication on PubMed
Search for more literature for 3.1.1.81

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.101
-
 N-(3-oxododecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
0.184
-
 N-(3-oxodecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
0.306
-
 L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
0.412
-
 N-butyryl-DL-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
0.526
-
 N-hexyl-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
0.608
-
 N-(3-oxooctyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta
-
-
-
Rhodococcus erythropolis
-
-
-
Rhodococcus erythropolis AC-1514D
-
-
-
Rhodococcus ruber
-
-
-
Rhodococcus ruber AC-1513D
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homoserine lactone + H2O
-
 Rhodococcus erythropolis L-homoserine
-
 ?
L-homoserine lactone + H2O
-
 Rhodococcus ruber L-homoserine
-
 ?
L-homoserine lactone + H2O
-
 Brevundimonas diminuta L-homoserine
-
 ?
additional information enzyme additionally displays organophosphate hydrolase activity, EC 3.1.8.1 Rhodococcus erythropolis ?
-
 ?
additional information enzyme additionally displays organophosphate hydrolase activity, EC 3.1.8.1 Rhodococcus ruber ?
-
 ?
additional information enzyme additionally displays organophosphate hydrolase activity, EC 3.1.8.1 Brevundimonas diminuta ?
-
 ?
N-(3-oxodecyl)-L-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-(3-oxodecyl)-L-homoserine
-
 ?
N-(3-oxodecyl)-L-homoserine lactone + H2O
-
 Rhodococcus ruber N-(3-oxodecyl)-L-homoserine
-
 ?
N-(3-oxodecyl)-L-homoserine lactone + H2O
-
 Brevundimonas diminuta N-(3-oxodecyl)-L-homoserine
-
 ?
N-(3-oxododecyl)-L-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-(3-oxododecyl)-L-homoserine
-
 ?
N-(3-oxododecyl)-L-homoserine lactone + H2O
-
 Rhodococcus ruber N-(3-oxododecyl)-L-homoserine
-
 ?
N-(3-oxododecyl)-L-homoserine lactone + H2O
-
 Brevundimonas diminuta N-(3-oxododecyl)-L-homoserine
-
 ?
N-(3-oxooctyl)-L-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-(3-oxooctyl)-L-homoserine
-
 ?
N-(3-oxooctyl)-L-homoserine lactone + H2O
-
 Rhodococcus ruber N-(3-oxooctyl)-L-homoserine
-
 ?
N-(3-oxooctyl)-L-homoserine lactone + H2O
-
 Brevundimonas diminuta N-(3-oxooctyl)-L-homoserine
-
 ?
N-butyryl-DL-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-butyryl-DL-homoserine
-
 ?
N-butyryl-DL-homoserine lactone + H2O
-
 Rhodococcus ruber N-butyryl-DL-homoserine
-
 ?
N-butyryl-DL-homoserine lactone + H2O
-
 Brevundimonas diminuta N-butyryl-DL-homoserine
-
 ?
N-hexyl-L-homoserine lactone + H2O
-
 Rhodococcus erythropolis N-hexyl-L-homoserine
-
 ?
N-hexyl-L-homoserine lactone + H2O
-
 Rhodococcus ruber N-hexyl-L-homoserine
-
 ?
N-hexyl-L-homoserine lactone + H2O
-
 Brevundimonas diminuta N-hexyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
OPH
-
 Brevundimonas diminuta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.83
-
 N-(3-oxododecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
2.62
-
 N-butyryl-DL-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
2.67
-
 N-(3-oxodecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
3.17
-
 N-hexyl-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
4.66
-
 N-(3-oxooctyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
4.69
-
 L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.35
-
 N-butyryl-DL-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
6.35
-
 N-hexyl-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
7.66
-
 N-(3-oxooctyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
14.5
-
 N-(3-oxodecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
15.3
-
 L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta
18.1
-
 N-(3-oxododecyl)-L-homoserine lactone pH 8.2, 25°C Brevundimonas diminuta