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Literature summary for 3.1.1.8 extracted from
- Comparison of efficiency of purification (from human plasma) of a nerve agent adduct of butyrylcholinesterase between the affinity gel method and immunomagnetic separation (2018), J. Chromatogr. Sci., 56, 248-253 .
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | O-ethyl S-2-diisopropylaminoethyl methyl phosphonothiolate (VX) forms phosphorylated adducts with enzyme BChE, inhibiting the enzyme. This adduct in human plasma can serve as a biomarker of exposure to nerve agents. Purification efficiency between the procainamide affinity gel method and immunomagnetic separation (IMS) for the nerve agent adduct of BChE in plasma is evaluated, the sample preparation is optimized by purifying BChE to measure biomarkers of human exposure to organophosphorus nerve agents. The purification efficiency of IMS is 5fold greater than that of the procainamide affinity gel method because the antibody conjugate with protein G magnetic beads ensures highly selective capture and high recovery of VX-inhibited BChE from plasma | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| O-ethyl S-2-diisopropylaminoethyl methyl phosphonothiolate | VX, a highly toxic chemical warfare agent, inhibition of butyrylcholinesterase (BChE) activity by VX is due to formation of a phosphorylated BChE adduct | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyrylcholine + H2O | Homo sapiens | - |
choline + butyrate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P06276 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| VX-inhibited BChE adducts from human plasma by the procainamide affinity gel method or the immunomagnetic separation (IMS) method. The purification efficiency of IMS is 5fold greater than that of the procainamide affinity gel method because the antibody conjugate with protein G magnetic beads ensures highly selective capture and high recovery of VX-inhibited BChE from plasma, overview | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyrylcholine + H2O | - |
Homo sapiens | choline + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | pepsin peptide mapping of VX-inhibited butyrylcholinesterase using liquid chromatography coupled to tandem mass spectrometry | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BChE | - |
Homo sapiens |
| butyrylcholinesterase | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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