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Literature summary for 3.1.1.79 extracted from
- Secreted lipases from Malassezia globosa recombinant expression and determination of their substrate specificities (2016), Microbiology, 162, 1069-1079 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene MGL_0797, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His7-tagged enzyme in Escherichia coli strain NEB10-beta, recombinant expression of His7-tagged enzyme in Pichia pastoris strain GS115 | Malassezia globosa |
| gene MGL_0799, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His7-tagged enzyme in Escherichia coli strain NEB10-beta, recombinant expression of His7-tagged enzyme in Pichia pastoris strain GS115 | Malassezia globosa |
| gene MGL_4054, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His7-tagged enzyme in Escherichia coli strain NEB10-beta, recombinant expression of His7-tagged enzyme in Pichia pastoris strain GS115 | Malassezia globosa |
| gene MGL_4197, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His7-tagged enzyme in Escherichia coli strain NEB10-beta, recombinant expression of His7-tagged enzyme in Pichia pastoris strain GS115 | Malassezia globosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Malassezia globosa | - |
- |
| additional information | the enzyme contains a 19 amino-acid signal peptide | Malassezia globosa | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malassezia globosa | A8PUY1 | - |
- |
| Malassezia globosa | A8PUY5 | - |
- |
| Malassezia globosa | A8QCW4 | - |
- |
| Malassezia globosa | A8QDF0 | - |
- |
| Malassezia globosa ATCC MYA-4612 | A8PUY1 | - |
- |
| Malassezia globosa ATCC MYA-4612 | A8PUY5 | - |
- |
| Malassezia globosa ATCC MYA-4612 | A8QCW4 | - |
- |
| Malassezia globosa ATCC MYA-4612 | A8QDF0 | - |
- |
| Malassezia globosa CBS 7966 | A8PUY1 | - |
- |
| Malassezia globosa CBS 7966 | A8PUY5 | - |
- |
| Malassezia globosa CBS 7966 | A8QCW4 | - |
- |
| Malassezia globosa CBS 7966 | A8QDF0 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | Lip1 contains two possible N-glycosylated asparagine residues, D205 and D253 | Malassezia globosa |
| glycoprotein | Lip2 contains two possible N-glycosylated asparagine residues, D98 and D230 | Malassezia globosa |
| glycoprotein | Lip3 contains two possible N-glycosylated asparagine residues, D98 and D230 | Malassezia globosa |
| glycoprotein | MDL2 contains three possible N-glycosylated asparagine residues, D102, D161, and D253 | Malassezia globosa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His7-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography | Malassezia globosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dilinolein + H2O | complete conversion | Malassezia globosa | monolineoylglycerol + linoleate | - |
? |  |
| dilinolein + H2O | over 50% conversion | Malassezia globosa | monolineoylglycerol + linoleate | - |
? | |
| dilinolein + H2O | complete conversion | Malassezia globosa CBS 7966 | monolineoylglycerol + linoleate | - |
? | |
| dilinolein + H2O | over 50% conversion | Malassezia globosa CBS 7966 | monolineoylglycerol + linoleate | - |
? | |
| dilinolein + H2O | complete conversion | Malassezia globosa ATCC MYA-4612 | monolineoylglycerol + linoleate | - |
? | |
| dilinolein + H2O | over 50% conversion | Malassezia globosa ATCC MYA-4612 | monolineoylglycerol + linoleate | - |
? | |
| dipalmitin + H2O | complete conversion | Malassezia globosa | monopalmitoylglycerol + palmitate | - |
? | |
| dipalmitin + H2O | complete conversion | Malassezia globosa CBS 7966 | monopalmitoylglycerol + palmitate | - |
? | |
| dipalmitin + H2O | complete conversion | Malassezia globosa ATCC MYA-4612 | monopalmitoylglycerol + palmitate | - |
? | |
| dipalmitolein + H2O | complete conversion | Malassezia globosa | monopalmitoleoylglycerol + palmitoleate | - |
? | |
| dipalmitolein + H2O | over 50% conversion | Malassezia globosa | monopalmitoleoylglycerol + palmitoleate | - |
? | |
| dipalmitolein + H2O | complete conversion | Malassezia globosa CBS 7966 | monopalmitoleoylglycerol + palmitoleate | - |
? | |
| dipalmitolein + H2O | over 50% conversion | Malassezia globosa CBS 7966 | monopalmitoleoylglycerol + palmitoleate | - |
? | |
| dipalmitolein + H2O | complete conversion | Malassezia globosa ATCC MYA-4612 | monopalmitoleoylglycerol + palmitoleate | - |
? | |
| dipalmitolein + H2O | over 50% conversion | Malassezia globosa ATCC MYA-4612 | monopalmitoleoylglycerol + palmitoleate | - |
? | |
| distearin + H2O | complete conversion | Malassezia globosa | monostearoylglycerol + stearate | - |
? | |
| distearin + H2O | over 50% conversion | Malassezia globosa | monostearoylglycerol + stearate | - |
? | |
| distearin + H2O | over 50% conversion | Malassezia globosa CBS 7966 | monostearoylglycerol + stearate | - |
? | |
| distearin + H2O | complete conversion | Malassezia globosa CBS 7966 | monostearoylglycerol + stearate | - |
? | |
| distearin + H2O | over 50% conversion | Malassezia globosa ATCC MYA-4612 | monostearoylglycerol + stearate | - |
? | |
| distearin + H2O | complete conversion | Malassezia globosa ATCC MYA-4612 | monostearoylglycerol + stearate | - |
? | |
| additional information | Lip2 substrate specificity, overview | Malassezia globosa | ? | - |
? | |
| additional information | Lip3 substrate specificity, overview | Malassezia globosa | ? | - |
? | |
| additional information | no activity of enzyme Lip1 with tristearin, trilinolein, and tripalmitoylglycerol. Lip1 substrate specificity, overview | Malassezia globosa | ? | - |
? | |
| additional information | no activity with dipalmitin, MDL2 substrate specificity, overview | Malassezia globosa | ? | - |
? | |
| additional information | no activity of enzyme Lip1 with tristearin, trilinolein, and tripalmitoylglycerol. Lip1 substrate specificity, overview | Malassezia globosa CBS 7966 | ? | - |
? | |
| additional information | no activity with dipalmitin, MDL2 substrate specificity, overview | Malassezia globosa CBS 7966 | ? | - |
? | |
| additional information | Lip2 substrate specificity, overview | Malassezia globosa CBS 7966 | ? | - |
? | |
| additional information | Lip3 substrate specificity, overview | Malassezia globosa CBS 7966 | ? | - |
? | |
| additional information | no activity of enzyme Lip1 with tristearin, trilinolein, and tripalmitoylglycerol. Lip1 substrate specificity, overview | Malassezia globosa ATCC MYA-4612 | ? | - |
? | |
| additional information | no activity with dipalmitin, MDL2 substrate specificity, overview | Malassezia globosa ATCC MYA-4612 | ? | - |
? | |
| additional information | Lip2 substrate specificity, overview | Malassezia globosa ATCC MYA-4612 | ? | - |
? | |
| additional information | Lip3 substrate specificity, overview | Malassezia globosa ATCC MYA-4612 | ? | - |
? | |
| trihexadecenoin + H2O | complete conversion | Malassezia globosa | dihexadecenoin + hexadecenoate | - |
? | |
| trihexadecenoin + H2O | below 30% conversion | Malassezia globosa | dihexadecenoin + hexadecenoate | - |
? | |
| trilinolein + H2O | complete conversion | Malassezia globosa | 1,2-dilinoleoylglycerol + oleate | - |
? | |
| tripalmitin + H2O | complete conversion | Malassezia globosa | 1,2-dipalmitoylglycerol + palmitate | - |
? | |
| tristearin + H2O | complete conversion | Malassezia globosa | 1,2-distearoylglycerol + stearate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33700, about, sequence calculation | Malassezia globosa |
| ? | x * 49400, about, sequence calculation | Malassezia globosa |
| ? | x * 49500, about, sequence calculation | Malassezia globosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Lip1 | - |
Malassezia globosa |
| LIP2 | - |
Malassezia globosa |
| LIP3 | - |
Malassezia globosa |
| MdL2 | - |
Malassezia globosa |
| MgLip2 | - |
Malassezia globosa |
| MgLip3 | - |
Malassezia globosa |
| MGL_0797 | - |
Malassezia globosa |
| MGL_0799 | - |
Malassezia globosa |
| MGL_4054 | - |
Malassezia globosa |
| MGL_4197 | - |
Malassezia globosa |
| MgMDL2 | - |
Malassezia globosa |
| secreted lipase | - |
Malassezia globosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Malassezia globosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Malassezia globosa |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | Lip1 is a family class 3 lipase. Family class 3 lipases are specific for mono- and diacylglycerols, but exhibit no regioselective production of diacylglycerols, in Malassezia globosa only LIP family lipases show activity on triacylglycerides. Comparisons of sequences and structures of Malassezia globosa lipases, overview | Malassezia globosa |
| evolution | Lip1 is a family class LIP lipase. Family class 3 lipases are specific for mono- and diacylglycerols, but exhibit no regioselective production of diacylglycerols, in Malassezia globosa only LIP family lipases show activity on triacylglycerides. Comparisons of sequences and structures of Malassezia globosa lipases, overview | Malassezia globosa |
| additional information | catalytic residues are Asp28 and His281 | Malassezia globosa |
| additional information | catalytic residues are Asp44 and His378 | Malassezia globosa |
| additional information | catalytic residues are Ser197, Asp44, and His378 | Malassezia globosa |
| physiological function | the enzyme plays a potential role in colonizing human skin | Malassezia globosa |
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