Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.79 extracted from

  • Ben Ali, Y.; Chahinian, H.; Petry, S.; Muller, G.; Carriere, F.; Verger, R.; Abousalham, A.
    Might the kinetic behavior of hormone-sensitive lipase reflect the absence of the lid domain? (2004), Biochemistry, 43, 9298-9306.
    View publication on PubMed
Search for more literature for 3.1.1.79

Cloned(Commentary)

Cloned (Comment) Organism
expression Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Diethyl p-nitrophenyl phosphate 100fold molar excess, complete inactivation after 15 min Homo sapiens
phenylmethylsulfonyl fluoride 100fold molar excess, complete inactivation after 15 min Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 tributanoyl glycerol 37°C, pH 8.0 Homo sapiens
10
-
 vinyl butyrate 37°C, pH 8.0 Homo sapiens
15
-
 tripropionyl glycerol 37°C, pH 8.0 Homo sapiens
70
-
 vinyl propionate 37°C, pH 8.0 Homo sapiens
170
-
 vinyl acetate 37°C, pH 8.0 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
lipid particle
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 2 * 84000, SDS-PAGE, recombinant HSL Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation of HSL leads to its translocation from the cytoplasm to the lipid droplet Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant HSL Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
143
-
 hydrolysis of vinyl butyrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dioleoyl glycerol + H2O 21% of activity with vinylbutyrate Homo sapiens monooleoylglycerol + oleate
-
 ?
olive oil + H2O 3% of activity with vinylbutyrate Homo sapiens ?
-
 ?
tributanoyl glycerol + H2O 8% of activity with vinylbutyrate Homo sapiens dibutanoylglycerol + butanoate
-
 ?
trioctanoyl glycerol + H2O 3% of activity with vinylbutyrate Homo sapiens dioctanoylglycerol + octanoate
-
 ?
tripropionyl glycerol + H2O 4% of activity with vinylbutyrate Homo sapiens dipropionylglycerol + propionate
-
 ?
vinyl acetate + H2O 67% of activity with vinylbutyrate Homo sapiens vinyl alcohol + acetate
-
 ?
vinyl butyrate + H2O
-
 Homo sapiens vinyl alcohol + butanoate
-
 ?
vinyl laurate + H2O 15% of activity with vinylbutyrate Homo sapiens vinyl alcohol + laurate
-
 ?
vinyl propionate + H2O 50% of activity with vinylbutyrate Homo sapiens vinyl alcohol + propionate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 84000, SDS-PAGE, recombinant HSL Homo sapiens

Synonyms

Synonyms Comment Organism
HSL
-
 Homo sapiens