Cloned (Comment) | Organism |
---|---|
expression in CHO cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
S423A | 99% loss of cholesteryl oleate hydrolysis, more than 80% loss of trioleoylglycerol hydrolysis and more than 90% loss of dioleoylglycerol hydrolysis | Rattus norvegicus |
S563A | 98% loss of cholesteryl oleate hydrolysis, more than 80% loss of trioleoylglycerol hydrolysis and more than 90% loss of dioleoylglycerol hydrolysis | Rattus norvegicus |
S563A/S565A | 70% loss of cholesteryl oleate hydrolysis, 10% loss of trioleoylglycerol hydrolysis and 80% loss of dioleoylglycerol hydrolysis | Rattus norvegicus |
S563D | approx. 20% increase in cholesteryl oleate hydrolysis compared to wild-type | Rattus norvegicus |
S565A | 70% loss of cholesteryl oleate hydrolysis, 35% loss of trioleoylglycerol hydrolysis and 30% loss of dioleoylglycerol hydrolysis | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
trioleoylglycerol | 37°C, pH 7.0 | Rattus norvegicus | |
0.0047 | - |
cholesteryl oleate | 37°C, pH 7.0 | Rattus norvegicus | |
0.03 | - |
dioleoylglycerol | 37°C, pH 7.0 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl oleate + H2O | approx. 55% of activity with dioleoylglcerol | Rattus norvegicus | cholesterol + oleate | - |
? | |
dioleoylglycerol + H2O | - |
Rattus norvegicus | monooleoylglycerol + oleate | - |
? | |
trioleoylglycerol + H2O | approx. 16% of activity with dioleoylglcerol | Rattus norvegicus | dioleoylglycerol + oleate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HSL | - |
Rattus norvegicus |