Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | 31% inhibition at 0.05 mM | Pseudomonas fluorescens | |
DTT | 38% inhibition at 0.05 mM | Pseudomonas putida | |
EDTA | 25% inhibition at 0.05 mM | Pseudomonas aeruginosa | |
KCN | 13% inhibition at 0.005 mM | Pseudomonas aeruginosa | |
KCN | 14% inhibition at 0.005 mM | Pseudomonas putida | |
additional information | no inhibition by DTT | Pseudomonas aeruginosa | |
additional information | no inhibition by KCN, PMSF, sodium azide, EDTA, and sodium deoxycholate | Pseudomonas fluorescens | |
additional information | no inhibition by sodium azide, EDTA, and sodium deoxycholate | Pseudomonas putida | |
PMSF | 23% inhibition at 0.05 mM | Pseudomonas aeruginosa | |
PMSF | 33% inhibition at 0.05 mM | Pseudomonas putida | |
Sodium azide | 30% inhibition at 0.05 mM | Pseudomonas aeruginosa | |
sodium deoxycholate | 35% inhibition at 0.5 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
poly(3-hydroxybutyrate) | pH 7.9, 30°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
K+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
Mg2+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
additional information | the enzyme requires divalent metal cations for activity | Pseudomonas fluorescens | |
additional information | the enzyme requires divalent metal cations for activity | Pseudomonas aeruginosa | |
additional information | the enzyme requires divalent metal cations for activity | Pseudomonas putida | |
Na+ | stimulates at 1 mM | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(3-hydroxybutyrate) + H2O | Pseudomonas fluorescens | - |
oligo(3-hydroxybutyrate) | - |
? | |
poly(3-hydroxybutyrate) + H2O | Pseudomonas aeruginosa | - |
oligo(3-hydroxybutyrate) | - |
? | |
poly(3-hydroxybutyrate) + H2O | Pseudomonas putida | - |
oligo(3-hydroxybutyrate) | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Pseudomonas fluorescens | co-polymeric substrate | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Pseudomonas aeruginosa | co-polymeric substrate | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Pseudomonas putida | co-polymeric substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
isolated from fuel-oil contaminated soil | - |
Pseudomonas fluorescens | - |
isolated from fuel-oil contaminated soil | - |
Pseudomonas putida | - |
isolated from fuel-oil contaminated soil | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas fluorescens |
additional information | - |
- |
Pseudomonas aeruginosa |
additional information | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(3-hydroxybutyrate) + H2O | - |
Pseudomonas fluorescens | oligo(3-hydroxybutyrate) | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Pseudomonas aeruginosa | oligo(3-hydroxybutyrate) | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Pseudomonas putida | oligo(3-hydroxybutyrate) | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | co-polymeric substrate | Pseudomonas fluorescens | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | co-polymeric substrate | Pseudomonas aeruginosa | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | co-polymeric substrate | Pseudomonas putida | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas fluorescens |
30 | - |
assay at | Pseudomonas aeruginosa |
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Pseudomonas fluorescens |
7.9 | - |
assay at | Pseudomonas aeruginosa |
7.9 | - |
assay at | Pseudomonas putida |