Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 5 mM, complete inhibition | Shewanella sp. JKCM-AJ-6,1A | |
Fe2+ | 1 mM less than 10% residual activity | Shewanella sp. JKCM-AJ-6,1A | |
NaCl | required for native enzyme, optimum activity at 0.5 M. Inhibitory for recombinant enzyme | Shewanella sp. JKCM-AJ-6,1A | |
PMSF | 5 mM, complete inhibition | Shewanella sp. JKCM-AJ-6,1A | |
SDS | 0.1%, complete inhibition | Shewanella sp. JKCM-AJ-6,1A | |
Tween 20 | 0.1%, complete inhibition | Shewanella sp. JKCM-AJ-6,1A |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | required for native enzyme, optimum activity at 0.5 M. Inhibitory for recombinant enzyme | Shewanella sp. JKCM-AJ-6,1A |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella sp. JKCM-AJ-6,1A | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant protein | Shewanella sp. JKCM-AJ-6,1A |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.34 | - |
native enzyme, pH 7.4, 15°C | Shewanella sp. JKCM-AJ-6,1A |
58.35 | - |
recombinant enzyme, pH 7.4, 15°C | Shewanella sp. JKCM-AJ-6,1A |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(3-hydroxybutanoate) + H2O | recombinant enzyme has a stronger binding affinity for poly(3-hydroxybutanoate) than native enzyme | Shewanella sp. JKCM-AJ-6,1A | ? | - |
? | |
poly(3-hydroxybutanoate-co-3-hydroxyvalerate) + H2O | 18 mol% hydroxyvalerate content | Shewanella sp. JKCM-AJ-6,1A | ? | - |
? | |
poly(ethylene succinate) + H2O | - |
Shewanella sp. JKCM-AJ-6,1A | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70382, calculated from sequence, x * 47000, native enzyme, x * 70000, recombinant enzyme, SDS-PAGE | Shewanella sp. JKCM-AJ-6,1A |
More | enzyme purified from Shewanella sp. lacks substrate binding domains which are present in the recombinant enzyme | Shewanella sp. JKCM-AJ-6,1A |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
stable up to | Shewanella sp. JKCM-AJ-6,1A |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Shewanella sp. JKCM-AJ-6,1A |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10.5 | - |
Shewanella sp. JKCM-AJ-6,1A |