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Literature summary for 3.1.1.75 extracted from

  • Muller, B.; Jendrossek, D.
    Purification and properties of poly(3-hydroxyvaleric acid) depolymerase from Pseudomonas lemoignei (1993), Appl. Microbiol. Biotechnol., 37-8, 487-492.
No PubMed abstract available
Search for more literature for 3.1.1.75

Inhibitors

Inhibitors Comment Organism Structure
2-propanol partial inhibition of P(3HV) depolymerase Paucimonas lemoignei
dithioerythritol complete inhibition of P(3HV) depolymerase at 4 mM, reversible by dithionitrobenzene Paucimonas lemoignei
EDTA partial inhibition of P(3HV) depolymerase at concentrations higher than 5 mM Paucimonas lemoignei
ethanol partial inhibition of P(3HV) depolymerase Paucimonas lemoignei
PMSF partial inhibition at 0.5 mM Paucimonas lemoignei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 poly(3-hydroxybutanoate)n 0.06 mg/ml, P(3HB) depolymerase B, 0.1 mg/ml, P(3HB) depolymerase A Paucimonas lemoignei
additional information
-
 poly(3-hydroxybutanoate)n values in mg/ml Paucimonas lemoignei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Paucimonas lemoignei
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 sucrose density gradient centrifugation Paucimonas lemoignei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Paucimonas lemoignei highest activities for poly(3-hydroxybutyrate) depolymerases A and B, low activity for poly(3-hydroxyvaleric acid) depolymerase poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Paucimonas lemoignei
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Paucimonas lemoignei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 19.1 mg poly[(R)-3-hydroxybutanoate]/min * mg protein, P(3HB) depolymerase A, 15.9 mg poly[(R)-3-hydroxybutanoate]/min * mg protein, P(3HB) depolymerase B, 9.27 mg poly(3-hydroxyvaleric acid)/min * mg protein Paucimonas lemoignei

Storage Stability

Storage Stability Organism
-20°C, P(3HV) depolymerase, 1 month Paucimonas lemoignei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxybutyric acid) + H2O
-
 Paucimonas lemoignei ?
-
 ?
poly(3-hydroxyvalerate)n + H2O highest activity for poly(3-hydroxyvaleric acid) depolymerase, low activity for poly(3-hydroxybutyrate) depolymerases A and B Paucimonas lemoignei poly(3-hydroxyvalerate)n-x + poly(3-hydroxyvalerate)
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O highest activities for poly(3-hydroxybutyrate) depolymerases A and B, low activity for poly(3-hydroxyvaleric acid) depolymerase Paucimonas lemoignei poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE Paucimonas lemoignei

Synonyms

Synonyms Comment Organism
P(3HB) depolymerase A
-
 Paucimonas lemoignei
P(3HB) depolymerase B
-
 Paucimonas lemoignei
P(3HV) depolymerase
-
 Paucimonas lemoignei
poly(3-hydroxyvaleric acid) depolymerase
-
 Paucimonas lemoignei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 P(3HV) depolymerase Paucimonas lemoignei
65
-
 P(3HB) depolymerase A Paucimonas lemoignei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 P(3HV) depolymerase: 25% loss of activity within 2 months Paucimonas lemoignei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 P(3HB) depolymerase A, P(3HV) depolymerase Paucimonas lemoignei