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Literature summary for 3.1.1.75 extracted from

  • Kasuya, K.; Inoue, Y.; Tanaka, T.; Akehata, T.; Iwata, T.; Fukui, T.; Doi, Y.
    Biochemical and molecular characterization of the polyhydroxybutyrate depolymerase of Comamonas acidovorans YM1609, isolated from freshwater (1997), Appl. Environ. Microbiol., 63, 4844-4852.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.75

Protein Variants

Protein Variants Comment Organism
additional information GST-catalytic domain fusion protein, high binding capacity on single crystals of poly(3-hydroxybutyrate) Delftia acidovorans

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate 90% inhibition at 10 mM Delftia acidovorans
dithiothreitol 83% inhibition at 10 mM Delftia acidovorans
PMSF 49% inhibition at 10 mM Delftia acidovorans
Tween 20 nearly complete inhibition at 0.1%, v/v Delftia acidovorans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Delftia acidovorans
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Delftia acidovorans
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x main products: dimer and monomer ?

Organism

Organism UniProt Comment Textmining
Delftia acidovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2step chromatography Delftia acidovorans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Delftia acidovorans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1 unit defined as the amount of protein required to decrease the value of A650 by 1.0 per minute Delftia acidovorans
additional information
-
 8U/mg Delftia acidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n + H2O 3-hydroxyvalerate composition can vary from 7-65 mol% Delftia acidovorans poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
-
 ?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n + H2O BIOPOL Delftia acidovorans poly(3-hydroxybutyrate-co-3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Delftia acidovorans poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x main products: dimer and monomer ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 sharp decrease of activity above Delftia acidovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Delftia acidovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 stable within Delftia acidovorans