Literature summary for 3.1.1.74 extracted from
Badenes, S.; Lemos, F.; Cabral, J.
Stability of cutinase, wild type and mutants, in AOT reversed micellar system-effect of mixture components of alkyl esters production (2011), J. Chem. Technol. Biotechnol., 86, 34-41.
No PubMed abstract available
Application
Application |
Comment |
Organism |
synthesis |
recombinant cutinase from Fusarium solani pisi is used as a catalyst in enzymatic transesterification between a mixture of triglyceride oils and methanol for biodiesel production in a bis(2-ethylhexyl) sodium sulfosuccinate (AOT)/isooctane reversed micellar system, kinetics, overview |
Fusarium vanettenii |
Protein Variants
Protein Variants |
Comment |
Organism |
L153Q |
site-directed mutagenesis, the mutant shows transesterification activity similar to the wild-type enzyme |
Fusarium vanettenii |
additional information |
cutinase is microencapsulated in reversed micelles of bis(2-ethylhexyl) sodium sulfosuccinate in isooctane for the production of alkyl esters, known as biodiesel, evaluation of the system stability using wild-type enzyme and three mutants, L153Q, T179C and S54D, method evaluation, overview. Loss of 45% of wild-type cutinase activity when incubated in the micellar system for 3 h, and an additional loss of 90% of the activity is observed in the presence of methanol after 10 min of incubation |
Fusarium vanettenii |
S54D |
site-directed mutagenesis, the mutant shows reduced transesterification activity compared to the wild-type enzyme |
Fusarium vanettenii |
T179C |
site-directed mutagenesis, the mutant shows transesterification activity similar to the wild-type enzyme, T179C displays high stability in the presence of methanol with an activity loss of only 16% as compared to 90% loss of wild-type activity, the mutant is also more stable microencapsulated in reversed micelles of bis(2-ethylhexyl) sodium sulfosuccinate in isooctane |
Fusarium vanettenii |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
glycerol |
inhibits the transesterification activity of the cutinase after 10 min of incubation |
Fusarium vanettenii |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Fusarium vanettenii |
- |
- |
- |