Application | Comment | Organism |
---|---|---|
synthesis | use of thermostable acetylxylan esterase from Thermobifida fusca and its synergistic action with xylanase for the production of xylooligosaccharides, overview. Cooperative enzymatic treatment of oat-spelt xylan by transformant xylanase and acetylxylan esterase | Thermobifida fusca |
Cloned (Comment) | Organism |
---|---|
gene axe, DNA and amino acid sequence determination and analysis, sequence comparisons, heterologous expression of His-tagged thermostable acetylxylan esterase gene in Escherichia coli | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
gene axe | - |
Thermobifida fusca NTU22 | - |
gene axe | - |
Purification (Comment) | Organism |
---|---|
recombinant thermostable His-tagged acetylxylan esterase from Escherichia coli by nickel affinity chromatography | Thermobifida fusca |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
109100 | - |
purified recombinant His-tagged enzyme, pH 7.5, 60°C | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | determination of xylanase activity by measuring the release of reducing sugars from oat-spelt xylan, production of xylobiose, xylotriose and xylotetrose by a crude enzyme extract. The purified recombinant His-tagged acetylxylan esterase has broad substrate specificity for various carboxyl esters. Among the polyoxyethylenesorbate esters tested, the enzyme has the highest activity for polyoxyethylenesorbate monolaurate, but moderate or little activity for longer acyl chains. The enzyme is able to hydrolyze both acetylxylan and acetylcellulose, but acetylxylan has a better hydrolytic rate | Thermobifida fusca | ? | - |
? | |
additional information | determination of xylanase activity by measuring the release of reducing sugars from oat-spelt xylan, production of xylobiose, xylotriose and xylotetrose by a crude enzyme extract. The purified recombinant His-tagged acetylxylan esterase has broad substrate specificity for various carboxyl esters. Among the polyoxyethylenesorbate esters tested, the enzyme has the highest activity for polyoxyethylenesorbate monolaurate, but moderate or little activity for longer acyl chains. The enzyme is able to hydrolyze both acetylxylan and acetylcellulose, but acetylxylan has a better hydrolytic rate | Thermobifida fusca NTU22 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant His-tagged enzyme | Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant His-tagged acetylxylan esterase, 3 h, 70% of maximal activity remaining | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant His-tagged enzyme | Thermobifida fusca |