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Literature summary for 3.1.1.72 extracted from
- Molecular cloning and characterization of a bifunctional xylanolytic enzyme from Neocallimastix patriciarum (2010), Appl. Microbiol. Biotechnol., 85, 1451-1462.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of a bifunctional acetylxylan esterase/xylanase, XynS20E, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli strain BL21 | Neocallimastix patriciarum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.72 | - |
birchwood xylan | pH 8.2, 58°C | Neocallimastix patriciarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neocallimastix patriciarum | B8YG19 | gene xynS20E | - |
| Neocallimastix patriciarum S20 | B8YG19 | gene xynS20E | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration | Neocallimastix patriciarum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 273.7 | - |
acetylxylan esterase activity on birchwood xylan, pH 8.2, 58°C, purified recombinant enzyme | Neocallimastix patriciarum |
| 557.9 | - |
acetylxylan esterase activity on oat spelt xylan, pH 8.2, 58°C, purified recombinant enzyme | Neocallimastix patriciarum |
| 580.3 | - |
acetylxylan esterase activity on 4-methylumbelliferyl acetate, pH 8.2, 58°C, purified recombinant enzyme | Neocallimastix patriciarum |
| 873.1 | - |
acetylxylan esterase activity on beechwood xylan, pH 8.2, 58°C, purified recombinant enzyme | Neocallimastix patriciarum |
| 1228 | - |
acetylxylan esterase activity on beta-D-xylose tetraacetate, pH 8.2, 58°C, purified recombinant enzyme | Neocallimastix patriciarum |
| 2614 | - |
acetylxylan esterase activity on acetylxylan, pH 8.2, 58°C, purified recombinant enzyme | Neocallimastix patriciarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl acetate + H2O | - |
Neocallimastix patriciarum | 4-methylumbelliferol + acetate | - |
? |  |
| 4-methylumbelliferyl acetate + H2O | - |
Neocallimastix patriciarum S20 | 4-methylumbelliferol + acetate | - |
? | |
| acetylxylan + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| acetylxylan + H2O | - |
Neocallimastix patriciarum S20 | ? | - |
? | |
| beechwood xylan + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| beechwood xylan + H2O | - |
Neocallimastix patriciarum S20 | ? | - |
? | |
| beta-D-xylose tetraacetate + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| birchwood xylan + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| additional information | the bifunctional acetylxylan esterase/xylanase shows xylanase and acetylxylan esterase activities on birchwood xylan | Neocallimastix patriciarum | ? | - |
? | |
| additional information | the bifunctional acetylxylan esterase/xylanase shows xylanase and acetylxylan esterase activities on birchwood xylan | Neocallimastix patriciarum S20 | ? | - |
? | |
| oat spelt xylan + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| oat spelt xylan + H2O | - |
Neocallimastix patriciarum S20 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | XynS20E contains a putative conserved domain of carbohydrate esterase family 1 at the N-terminus and a putative conserved domain of glycosyl hydrolase family 11 at the C-terminus | Neocallimastix patriciarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bifunctional acetylxylan esterase/xylanase | - |
Neocallimastix patriciarum |
| XynS20E | - |
Neocallimastix patriciarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
carbohydrate esterase activity | Neocallimastix patriciarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
carbohydrate esterase activity | Neocallimastix patriciarum |
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Release 2023.1 (January 2023)
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