Any feedback?
Literature summary for 3.1.1.58 extracted from
- A pathway of polygalactosamine formation in Aspergillus parasiticus: enzymatic deacetylation of N-acetylated polygalactosamine (1979), Eur. J. Biochem., 102, 35-42.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates, optimal concentration: 80 mM, pH 9.3: inhibition | Aspergillus parasiticus |  |
| Co2+ | pH 5.3: stimulates, optimal concentration: 80 mM, pH 9.3: inhibition | Aspergillus parasiticus | |
| Mg2+ | pH 5.3: stimulates, optimal concentration: 80 mM, pH 9.3: inhibition | Aspergillus parasiticus | |
| Mn2+ | pH 5.3: stimulates, optimal concentration: 80 mM, pH 9.3: inhibition | Aspergillus parasiticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
N-acetylated polygalactosamine | 30°C, pH 5.3 and pH 9.3 | Aspergillus parasiticus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Aspergillus oryzae | - |
- |
| soluble | - |
Aspergillus parasiticus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates, optimal concentration: 80 mM, pH 9.3: inhibition | Aspergillus parasiticus | |
| Co2+ | pH 5.3: stimulates, optimal concentration: 80 mM, pH 9.3: inhibition | Aspergillus parasiticus | |
| Mg2+ | pH 5.3: stimulates, optimal concentration: 80 mM, pH 9.3: inhibition | Aspergillus parasiticus | |
| Mn2+ | pH 5.3: stimulates, optimal concentration: 80 mM, pH 9.3: inhibition | Aspergillus parasiticus | |
| molybdate | ammonium molybdate, pH 5.3: maximal stimulation at 2.5 mM, pH 9.3: slight stimulation | Aspergillus parasiticus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetylgalactosaminoglycan + H2O | Aspergillus parasiticus | occurrence of this enzyme may account for the formation of the polygalactosamine with amino groups | galactosaminoglycan + acetate | - |
? | |
| N-acetylgalactosaminoglycan + H2O | Aspergillus parasiticus AHU 7165 | occurrence of this enzyme may account for the formation of the polygalactosamine with amino groups | galactosaminoglycan + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
AHU 7139 | - |
| Aspergillus oryzae AHU 7139 | - |
AHU 7139 | - |
| Aspergillus parasiticus | - |
AHU 7165 | - |
| Aspergillus parasiticus AHU 7165 | - |
AHU 7165 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 140fold, AHU 7165 | Aspergillus parasiticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.529 | - |
- |
Aspergillus parasiticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, stable for several months | Aspergillus parasiticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no substrates: bacterial cell wall peptidoglycan, N-acetylated heparin, partially O-hydroxyethylated chitin or monomeric N-acetylgalactosamine derivatives | Aspergillus parasiticus | ? | - |
? | |
| additional information | no substrates: bacterial cell wall peptidoglycan, N-acetylated heparin, partially O-hydroxyethylated chitin or monomeric N-acetylgalactosamine derivatives | Aspergillus parasiticus AHU 7165 | ? | - |
? | |
| N-acetylated oligogalactosamine + H2O | rate of deacetylation increases with the number of monosaccharide units in the oligosaccharides: with 14 or more N-acetylgalactosamine units: at a rate similar to that of deacetylation of the polymer, trimer to hexamer: slowly, inactive towards disaccharide | Aspergillus parasiticus | ? | - |
? | |
| N-acetylated oligogalactosamine + H2O | rate of deacetylation increases with the number of monosaccharide units in the oligosaccharides: with 14 or more N-acetylgalactosamine units: at a rate similar to that of deacetylation of the polymer, trimer to hexamer: slowly, inactive towards disaccharide | Aspergillus parasiticus AHU 7165 | ? | - |
? | |
| N-acetylated polygalactosamine + H2O | - |
Aspergillus parasiticus | polygalactosamine + acetate | - |
? | |
| N-acetylated polygalactosamine + H2O | - |
Aspergillus parasiticus AHU 7165 | polygalactosamine + acetate | - |
? | |
| N-acetylgalactosaminoglycan + H2O | - |
Aspergillus oryzae | galactosaminoglycan + acetate | - |
? | |
| N-acetylgalactosaminoglycan + H2O | - |
Aspergillus parasiticus | galactosaminoglycan + acetate | - |
? | |
| N-acetylgalactosaminoglycan + H2O | occurrence of this enzyme may account for the formation of the polygalactosamine with amino groups | Aspergillus parasiticus | galactosaminoglycan + acetate | - |
? | |
| N-acetylgalactosaminoglycan + H2O | - |
Aspergillus oryzae AHU 7139 | galactosaminoglycan + acetate | - |
? | |
| N-acetylgalactosaminoglycan + H2O | - |
Aspergillus parasiticus AHU 7165 | galactosaminoglycan + acetate | - |
? | |
| N-acetylgalactosaminoglycan + H2O | occurrence of this enzyme may account for the formation of the polygalactosamine with amino groups | Aspergillus parasiticus AHU 7165 | galactosaminoglycan + acetate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
and pH 9.3, 2 optima | Aspergillus parasiticus |
| 9.3 | - |
and pH 5.3, 2 optima | Aspergillus parasiticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
