Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activity is affected considerably, 5.0 mg/ml prevents substrate polymerization | Tagetes patula |
General Stability | Organism |
---|---|
after 6 months of repeated thawing and freezing about 50% of the esterase activity disappears | Tagetes patula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | BSA forms a weak conjugate with the substrate which inhibits the enzyme competitively | Tagetes patula | |
additional information | ascorbic acid, KCN at 0.001-100 mM, cysteine, glutathione, dithiothreitol, 5,5'-dithiobis(2-nitrobenzoate), 4-chloromercuribenzoate at 0.001-1 mM are no inhibitors | Tagetes patula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
5-(4-Acetoxybut-1-ynyl)-2,2'-bithiophene | pH 8.0, 30°C | Tagetes patula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Tagetes patula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O | Tagetes patula | enzyme involved in the transformation of an acetylenic compound | 5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tagetes patula | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Tagetes patula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Tagetes patula | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0156 | - |
- |
Tagetes patula |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme can be stored for more than 3 months without a marked loss of activity | Tagetes patula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O | - |
Tagetes patula | 5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate | - |
? | |
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O | enzyme involved in the transformation of an acetylenic compound | Tagetes patula | 5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate | - |
? | |
additional information | none of various acetate esters as well as other naturally occuring esters like chlorogenic acid or p-coumaroyl-CoA are hydrolysed by the enzyme | Tagetes patula | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
preparation heated for 5 min is inactive | Tagetes patula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Tagetes patula |