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Literature summary for 3.1.1.53 extracted from
- O-Acetylation and de-O-acetylation of sialic acids. Purification, characterization, and properties of a glycosylated rat liver esterase specific for 9-O-acetylated sialic acids (1989), J. Biol. Chem., 264, 19435-19442.
General Stability
| General Stability | Organism |
|---|---|
| lyophilization and reconstitution results in loss of 30% of activity | Rattus norvegicus |
| single cycle of freeze-thaw results in little loss of activity, repeated freeze-thaw results in gradual loss of activity | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Butanedione | irreversibly inactivated | Rattus norvegicus |  |
| 9-acetamido-N-acetyl-O-acetylneuraminate | no effect up to 5 mM | Rattus norvegicus | |
| bis-p-nitrophenyl phosphate | little effect on the activity | Rattus norvegicus | |
| CHAPS | lowers the activity by 40% | Rattus norvegicus | |
| deoxycholate | 75-80% loss of activity at 2% concentration | Rattus norvegicus | |
| Diethyl-4-nitrophenylphosphate | irreversibly inactivated | Rattus norvegicus | |
| diisopropyl fluorophosphate | irreversibly inactivated | Rattus norvegicus | |
| F- | little effect on the activity | Rattus norvegicus | |
| Hg2+ | little effect on the activity | Rattus norvegicus | |
| additional information | not affected by detergents, i.e. Triton X-100, Triton CF-54, saponin; not affected by EDTA | Rattus norvegicus | |
| p-chloromercuribenzoate | little effect on the activity | Rattus norvegicus | |
| Phenylglyoxal | irreversibly inactivated | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.8 | - |
N-acetyl-9-O-acetylneuraminic acid | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no divalent cation requirement | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36400 | - |
1 * 36400 + 1* 30300, SDS-PAGE | Rattus norvegicus |
| 62800 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | O-acetyl esters | ? | - |
? | |
| additional information | Rattus norvegicus | specific for sialic acids and selectively cleaves acetyl groups at the 9-position. it deacetylates di-O-acetyl- and tri-O-acetyl-N-acetylneuraminic acids by first cleaving the O-acetyl ester at the 9-position. The 7- and 8-acetylesters then undergo spontaneous migration to the 9-position, where they can be cleaved | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | contains N-linked oligosaccarides, after treatment with peptide N-glycosidase decrease of 10000 Da in the apparent molecular weight, endoglycosidase H causes a much smaller shift | Rattus norvegicus |
| side-chain modification | glycoprotein, linked with N-oligosaccharides, LSE, cleaved by endo-beta-N-acetylglycosaminidase H and peptide:N-glycosidase F | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3000fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | almost exclusively the nonglycosylated form of the enzyme | Rattus norvegicus | - |
| colonic mucosa | high levels of activity of both forms of the enzyme, nonglycosylated and glycosylated | Rattus norvegicus | - |
| heart | almost exclusively the nonglycosylated form of the enzyme | Rattus norvegicus | - |
| kidney | high levels of activity of both forms, nonglycosylated and glycosylated | Rattus norvegicus | - |
| liver | acetone powder, equal amounts of the glycosylated and nonglycosylated form of the enzyme | Rattus norvegicus | - |
| skeletal muscle | only the glycosylated form of the enzyme | Rattus norvegicus | - |
| testis | high levels of activity of both forms, nonglycosylated and glycosylated | Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, concentrated purified enzyme, stable for at least 1 month | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-O-acetylthymidine + H2O | 8% of the activity compared to N-acetyl-9-O-acetylneuraminate | Rattus norvegicus | thymidine + acetate | - |
? | |
| 4-methylumbelliferyl butyrate + H2O | less than 5% of the activity compared to 4-methylumbelliferyl acetate at pH 5.5 | Rattus norvegicus | 4-methylumbelliferone + butyrate | - |
? | |
| 4-nitrophenyl acetate + H2O | 72% of the activity compared to 4-methylumbelliferyl acetate at pH 5.5 | Rattus norvegicus | 4-nitrophenol + acetate | - |
? | |
| alpha-naphthyl acetate + H2O | 65% of the activity compared to 4-methylumbelliferyl acetate at pH 5.5 | Rattus norvegicus | 1-naphthol + acetate | - |
? | |
| additional information | strict substrate specificity for O-acetylated acids and a broad specificity for small synthetic esters, little or no activity against natural acetylated compounds other than sialic acids | Rattus norvegicus | ? | - |
? | |
| additional information | O-acetyl esters | Rattus norvegicus | ? | - |
? | |
| additional information | specific for sialic acids and selectively cleaves acetyl groups at the 9-position. it deacetylates di-O-acetyl- and tri-O-acetyl-N-acetylneuraminic acids by first cleaving the O-acetyl ester at the 9-position. The 7- and 8-acetylesters then undergo spontaneous migration to the 9-position, where they can be cleaved | Rattus norvegicus | ? | - |
? | |
| N-acetyl-9-O-acetylneuraminate + H2O | - |
Rattus norvegicus | N-acetylneuraminate + acetate | - |
? | |
| O-acetylserine + H2O | less than 5% of the activity compared to N-acetyl-9-O-acetylneuraminate | Rattus norvegicus | serine + acetate | - |
? | |
| triacetin + H2O | 26% of the activity compared to N-acetyl-9-O-acetylneuraminate | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 36400 + 1* 30300, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sialic acid O-acetylesterase | - |
Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
40% activity is lost in 20 h | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5: 16% of the activity, pH 8: 80% of the activity | Rattus norvegicus |
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Release 2023.1 (January 2023)
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