Protein Variants | Comment | Organism |
---|---|---|
additional information | the surface mutations Q110L, Y137C and N154D do not influence the kinetic properties. Mutations Q110L and N154D have stabilizing effects, the Y137C mutation alone is destabilizing. The three mutations together increase the melting temperature by 3.4 degrees | Pseudomonas knackmussii |
Q35H/F38L/C123S/Y137C/Y145C/E199G/S208G/G211D | mutations do not influence the kinetic properties | Pseudomonas knackmussii |
Q35H/F38L/C123S/Y145C/E199G/S208G/G211D | mutations do not influence the kinetic properties | Pseudomonas knackmussii |
Q35H/F38L/C123S/Y145C/N154D/E199G/S208G/G211D | mutations do not influence the kinetic properties | Pseudomonas knackmussii |
Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D | mutations do not influence the kinetic properties | Pseudomonas knackmussii |
Q35H/F38L/Q110L/C123S/Y145C/E199G/S208G/G211D | mutations do not influence the kinetic properties | Pseudomonas knackmussii |
Q35H/F38L/Q110L/C123S/Y145C/N154D/E199G/S208G/G211D | mutations do not influence the kinetic properties | Pseudomonas knackmussii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.013 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/C123S/Y137C/Y145C/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.013 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.013 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/Q110L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.014 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.014 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/Q110L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas knackmussii | P0A115 | - |
- |
Pseudomonas knackmussii DSM 6978 | P0A115 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Pseudomonas knackmussii | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Pseudomonas knackmussii DSM 6978 | 4-nitrophenol + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ClcD | - |
Pseudomonas knackmussii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51.5 | - |
melting temperature, mutant Q35H/F38L/C123S/Y145C/E199G/S208G/G211D | Pseudomonas knackmussii |
54.9 | - |
melting temperature, mutant Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D | Pseudomonas knackmussii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.57 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/C123S/Y137C/Y145C/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.58 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.58 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/Q110L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.59 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.6 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/Q110L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
0.61 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/C123S/Y137C/Y145C/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
43 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/Q110L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
44 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/Q110L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
45 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/Q110L/C123S/Y137C/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
46 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/C123S/Y145C/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii | |
49 | - |
4-nitrophenyl acetate | mutant Q35H/F38L/C123S/Y145C/N154D/E199G/S208G/G211D, pH 7.5, 25°C | Pseudomonas knackmussii |