Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(p-amino-phenyl)methanesulfonyl fluoride | slight inhibition | Rhodotorula glutinis | |
5,5'-dithiobis(2-nitrobenzoate) | - |
Rhodotorula glutinis | |
Cu2+ | CuCl2 | Rhodotorula glutinis | |
eserine sulfate | slight inhibition | Rhodotorula glutinis | |
Hg2+ | HgCl2 | Rhodotorula glutinis | |
PbCl2 | - |
Rhodotorula glutinis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.11 | - |
benzyloxycarbonyl-7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
1.43 | - |
7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
2.03 | - |
monochloroacetyl-7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
8.39 | - |
cephalosporin C | - |
Rhodotorula glutinis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | the majority of the enzyme is tightly bound to the cell wall | Rhodotorula glutinis | 5618 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
2 * 82000, SDS-PAGE | Rhodotorula glutinis |
141000 | - |
gel filtration | Rhodotorula glutinis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula glutinis | - |
- |
- |
Rhodotorula glutinis 38B1 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | N-linked sugar content is about 21% by weight | Rhodotorula glutinis |
Purification (Comment) | Organism |
---|---|
- |
Rhodotorula glutinis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
342 | - |
- |
Rhodotorula glutinis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis 38B1 | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
benzyloxycarbonyl-7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis | ? | - |
? | |
benzyloxycarbonyl-7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis 38B1 | ? | - |
? | |
cephalosporin C + H2O | - |
Rhodotorula glutinis | deacetylcephalosporin C + acetate | - |
? | |
cephalosporin C + H2O | - |
Rhodotorula glutinis 38B1 | deacetylcephalosporin C + acetate | - |
? | |
monochloroacetyl-7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis | acetate + monochloroacetyl-deacetyl-7-aminocephalosporanic acid | - |
? | |
monochloroacetyl-7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis 38B1 | acetate + monochloroacetyl-deacetyl-7-aminocephalosporanic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 82000, SDS-PAGE | Rhodotorula glutinis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Rhodotorula glutinis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 45 | 10°C: about 40% of maximal activity, 45°C: about 30% of maximal activity | Rhodotorula glutinis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
15 min, 60% loss of activity | Rhodotorula glutinis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
534 | - |
benzyloxycarbonyl-7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
576 | - |
7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
768 | - |
monochloroacetyl-7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
1370 | - |
cephalosporin C | - |
Rhodotorula glutinis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Rhodotorula glutinis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 7 | pH 4.2: 30% of maximal activity, pH 7.0: about 35% of maximal activity | Rhodotorula glutinis |