Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | slight increase in phospholipase activity during the summer | Hexaplex trunculus | |
sodium deoxycholate | is required for activity, maximum activity above 4 mM | Hexaplex trunculus | |
sodium taurodeoxycholate | is required for activity, maximum activity above 4 mM | Hexaplex trunculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Hexaplex trunculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | specific activity increases to reach its maximum in the presence of 10 mM Ca2+ using phosphatidylcholine as substrate | Hexaplex trunculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Hexaplex trunculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camelus dromedarius | - |
- |
- |
Hexaplex trunculus | - |
- |
- |
Struthio camelus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Camelus dromedarius |
- |
Struthio camelus |
by ammonium sulfate and ethanol precipitation and gel filtration, to homogeneity, 250fold. Overall recovery of phospholipase activity is about 82% | Hexaplex trunculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatopancreas | - |
Hexaplex trunculus | - |
pancreas | - |
Sus scrofa | - |
pancreas | - |
Camelus dromedarius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.72 | - |
crude extract, at 50°C and pH 8.5, with phosphatidylcholine liposomes as a substrate in the presence of 4 mM sodium taurodeoxycholate and 6 mM CaCl2 | Hexaplex trunculus |
180 | - |
250fold purified enzyme, at 50°C and pH 8.5, with phosphatidylcholine liposomes as a substrate in the presence of 4 mM sodium taurodeoxycholate and 6 mM CaCl2 | Hexaplex trunculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-stearoyl-2-oleoyl-3-sn-glycerophosphorylcholine + H2O | - |
Hexaplex trunculus | ? | - |
? | |
egg yolk + H2O | - |
Hexaplex trunculus | ? | - |
? | |
phosphatidylcholine + H2O | - |
Hexaplex trunculus | 1-acylglycerophosphocholine + fatty acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Hexaplex trunculus |
Synonyms | Comment | Organism |
---|---|---|
marine snail digestive phospholipase A2 | - |
Hexaplex trunculus |
mSDPL | - |
Hexaplex trunculus |
phospholipase A2 | - |
Sus scrofa |
phospholipase A2 | - |
Camelus dromedarius |
phospholipase A2 | - |
Struthio camelus |
phospholipase A2 | - |
Hexaplex trunculus |
PLA2 | - |
Sus scrofa |
PLA2 | - |
Camelus dromedarius |
PLA2 | - |
Struthio camelus |
PLA2 | - |
Hexaplex trunculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Hexaplex trunculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | - |
Hexaplex trunculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
retains more than 80% or 40% of its initial activity after 30 min of incubation at 40 or 50°C, respectively. The enzyme is completely inactivated after incubation at 55°C during 30 min. Presence of substrate increases thermostability | Hexaplex trunculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Hexaplex trunculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Hexaplex trunculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | is highly stable over a broad pH range, maintains over 100% of its maximal activity between pH 7.0 and 9.0 and 60% at pH 10. The enzyme is completely inactivated at pH 4.0 for 30 min | Hexaplex trunculus |
General Information | Comment | Organism |
---|---|---|
physiological function | does not show any hemolysis activity in intact erythrocytes | Sus scrofa |
physiological function | does not show any hemolysis activity in intact erythrocytes. Is active at low surface pressure | Camelus dromedarius |
physiological function | does not show any hemolysis activity in intact erythrocytes. Is active at low surface pressure | Struthio camelus |
physiological function | exhibits hemolytic activity in intact erythrocytes and can penetrate phospholipid monolayers at high surface pressure. May be toxic to mammal cells | Hexaplex trunculus |