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Literature summary for 3.1.1.4 extracted from

  • Ishimaru, K.; Kihara, H.; Ohno, M.
    Purification and properties of phospholipase A2 from venom of Trimeresurus flavoviridis (Habu snake) (1980), J. Biochem., 88, 443-451.
    View publication on PubMed
Search for more literature for 3.1.1.4

Inhibitors

Inhibitors Comment Organism Structure
N-bromosuccinimide
-
 Protobothrops flavoviridis
p-bromophenacyl bromide
-
 Protobothrops flavoviridis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 SDS-PAGE Protobothrops flavoviridis
28000
-
 gel filtration Protobothrops flavoviridis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Protobothrops flavoviridis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Protobothrops flavoviridis
-
 Habu snake
-

Oxidation Stability

Oxidation Stability Organism
oxidation with N-bromosuccinimide at pH 4, complete loss of activity when two Trp residues per subunit are oxidized Protobothrops flavoviridis

Purification (Commentary)

Purification (Comment) Organism
ion-exchange chromatography, gel-filtration, 11fold Protobothrops flavoviridis

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Protobothrops flavoviridis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1300
-
 egg yolk lipoprotein assay Protobothrops flavoviridis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 Protobothrops flavoviridis 1-acylglycerophosphocholine + fatty acid
-
 ?
phospholipids + H2O
-
 Protobothrops flavoviridis ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 14000, identical subunits, gel filtration, SDS-PAGE Protobothrops flavoviridis