Activating Compound | Comment | Organism | Structure |
---|---|---|---|
serine | human serine dependent PLA2 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into baculovirus, transfection of SF9 insect cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | - |
Homo sapiens | |
Diethyl p-nitrophenyl phosphate | - |
Homo sapiens | |
diisopropyl fluorophosphate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
1-decanoyl-2-(4-nitrophenylglutaryl) phosphate | - |
Homo sapiens | |
0.042 | - |
platelet activating factor | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44160 | - |
SDS-PAGE and liquid chromatography | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospholipids + H2O | Homo sapiens | influence on platelet aggregation | ? | - |
? | |
platelet activating factor + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
leukocyte | peripheral blood | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35 | - |
HSD-PLA2, 21fold purification, platelet activating factor for substrate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-decanoyl-2-(4-nitrophenylglutaryl)glycerol 3-phosphate + H2O | HSD-PLA2 | Homo sapiens | ? | - |
? | |
oxidized phosphatidylcholines + H2O | HSD-PLA2 | Homo sapiens | 1-acylglycerophosphocholine + oxidized fatty acids | - |
? | |
phospholipids + H2O | influence on platelet aggregation | Homo sapiens | ? | - |
? | |
platelet activating factor + H2O | - |
Homo sapiens | ? | - |
? | |
platelet activating factor + H2O | PAF i.e. platelet activating factor, HSD-PLA2 | Homo sapiens | ? | - |
? |