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Literature summary for 3.1.1.35 extracted from
- Role of Acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids (2003), Eur. J. Biochem., 270, 486-494.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Acinetobacter calcoaceticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
peracetic acid | 30°C, pH 5.0 | Acinetobacter calcoaceticus |  |
| 0.8 | - |
3,4-dihydrocoumarin | 30°C, pH 7.0 | Acinetobacter calcoaceticus | |
| 25.9 | - |
DL-beta-acetylthioisobutyrate methyl ester | 30°C, pH 7.0 | Acinetobacter calcoaceticus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrocoumarin + H2O | Acinetobacter calcoaceticus | - |
melilotate | - |
? | |
| dihydrocoumarin + H2O | Acinetobacter calcoaceticus F46 | - |
melilotate | - |
? | |
| peracetic acid | Acinetobacter calcoaceticus | peracetic acid is likely a natural substrate in vivo, enzyme may play a role in oxidative stress defense system | acetic acid + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | Q83WC8 | - |
- |
| Acinetobacter calcoaceticus F46 | Q83WC8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.3 | - |
monochlordimedonbrominating activity, recombinant enzyme | Acinetobacter calcoaceticus |
| 116 | - |
3,4-dihydrocoumarin hydrolysis, recombinant enzyme | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloro-5,5-dimethyl-1,3-cyclohexanedimedon + Br- + peracetic acid | - |
Acinetobacter calcoaceticus | 2-chloro-2-bromo-5,5-dimethyl-1,3-cyclohexanedimedon + acetic acid + H2O | - |
? | |
| 2-chloro-5,5-dimethyl-1,3-cyclohexanedimedon + Br- + peracetic acid | - |
Acinetobacter calcoaceticus F46 | 2-chloro-2-bromo-5,5-dimethyl-1,3-cyclohexanedimedon + acetic acid + H2O | - |
? | |
| 3,4-dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus | 3-(2-hydroxyphenyl)propionic acid | - |
? | |
| 3,4-dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus F46 | 3-(2-hydroxyphenyl)propionic acid | - |
? | |
| dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus | melilotate | - |
? | |
| dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus F46 | melilotate | - |
? | |
| DL-beta-acetylthioisobutyrate methyl ester + H2O | stereospecific hydrolysis | Acinetobacter calcoaceticus | D-beta-acetylthioisobutyrate + methanol | - |
? | |
| DL-beta-acetylthioisobutyrate methyl ester + H2O | stereospecific hydrolysis | Acinetobacter calcoaceticus F46 | D-beta-acetylthioisobutyrate + methanol | - |
? | |
| peracetic acid | peracetic acid is likely a natural substrate in vivo, enzyme may play a role in oxidative stress defense system | Acinetobacter calcoaceticus | acetic acid + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3,4-dihydrocoumarin hydrolase | - |
Acinetobacter calcoaceticus |
| DCH | - |
Acinetobacter calcoaceticus |
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