Cloned (Comment) | Organism |
---|---|
gene lip9, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) inclusion bodies, subcloning in Escherichia coli strain JM109 | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | LST-03 lipase is secreted | Pseudomonas aeruginosa | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | A8QYB2 | LST-03 lipase; gene lip9 | - |
Pseudomonas aeruginosa LST-03 | A8QYB2 | LST-03 lipase; gene lip9 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, after solubilization from inclusion bodies, by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration to homogeneity | Pseudomonas aeruginosa |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli inclusion bodies by 20 mM Tris-HCl buffer, pH 8.0, and 5.3 M urea, followed by a 100fold dilution | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dimercaptopropan-1-ol tributyrate + H2O | liberation of thiol groups by hydrolysis | Pseudomonas aeruginosa | ? | - |
? | |
2,3-dimercaptopropan-1-ol tributyrate + H2O | liberation of thiol groups by hydrolysis | Pseudomonas aeruginosa LST-03 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lip9 | - |
Pseudomonas aeruginosa |
LST-03 lipase | - |
Pseudomonas aeruginosa |
organic solvent-stable lipase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |