Cloned (Comment) | Organism |
---|---|
construction of a genetic library from genomic DNA, P1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli M15 | Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
stable for 1 h at 37°C in 0.1% CHAPS and Triton X-100 | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-hexandecanesulfonyl chloride | complete inhibition at 10 mM | Geobacillus stearothermophilus | |
2-mercaptoethanol | slight inhibition | Geobacillus stearothermophilus | |
CHAPS | inhibits at 1% | Geobacillus stearothermophilus | |
Cs+ | slight inhibition | Geobacillus stearothermophilus | |
dithiothreitol | - |
Geobacillus stearothermophilus | |
dodecanesulfonyl chloride | - |
Geobacillus stearothermophilus | |
EDTA | - |
Geobacillus stearothermophilus | |
Fe2+ | 47% inhibition at 1 mM, 99.2% inhibition at 10 mM | Geobacillus stearothermophilus | |
K+ | slight inhibition | Geobacillus stearothermophilus | |
Li+ | slight inhibition | Geobacillus stearothermophilus | |
Mn2+ | slight inhibition | Geobacillus stearothermophilus | |
PMSF | strong inhibition at 10 mM | Geobacillus stearothermophilus | |
SDS | inhibits at 0.1% | Geobacillus stearothermophilus | |
sodium deoxycholate | inhibits at 1% | Geobacillus stearothermophilus | |
Triton X-100 | inhibits at 1% | Geobacillus stearothermophilus | |
Tween 20 | inhibits at 1% | Geobacillus stearothermophilus | |
Zn2+ | 65% inhibition at 1 mM, 98.4% inhibition at 10 mM | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tricaprylin + H2O | Geobacillus stearothermophilus | - |
dicaprylin + caprylate | - |
? | |
tricaprylin + H2O | Geobacillus stearothermophilus P1 | - |
dicaprylin + caprylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q9L6D3 | - |
- |
Geobacillus stearothermophilus P1 | Q9L6D3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, 18fold, to homogeneity in a one-step procedure | Geobacillus stearothermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | enzyme contains an active site serine | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
811 | - |
purified recombinant enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl caprylate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Geobacillus stearothermophilus P1 | 4-nitrophenol + caprylate | - |
? | |
additional information | substrate specificity, overview | Geobacillus stearothermophilus | ? | - |
? | |
additional information | substrate specificity, overview | Geobacillus stearothermophilus P1 | ? | - |
? | |
tricaprylin + H2O | - |
Geobacillus stearothermophilus | dicaprylin + caprylate | - |
? | |
tricaprylin + H2O | best substrate | Geobacillus stearothermophilus | dicaprylin + caprylate | - |
? | |
tricaprylin + H2O | - |
Geobacillus stearothermophilus P1 | dicaprylin + caprylate | - |
? | |
tricaprylin + H2O | best substrate | Geobacillus stearothermophilus P1 | dicaprylin + caprylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000-43209, recombinant enzyme, SDS-PAGE and mass spectrometry | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme | Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | - |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | highly stable | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant enzyme | Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | - |
Geobacillus stearothermophilus |