Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | strong inhibition | Micrococcus sp. | |
Co2+ | - |
Micrococcus sp. | |
Cu2+ | - |
Micrococcus sp. | |
cysteine | - |
Micrococcus sp. | |
dithiothreitol | strong inhibition | Micrococcus sp. | |
EDTA | strong inhibition | Micrococcus sp. | |
Fe2+ | - |
Micrococcus sp. | |
Mn2+ | slight inhibition | Micrococcus sp. | |
additional information | no inhibition by PMSF, E-64, 1,10-phenanthroline, and tosyllysylchloromethyl ketone | Micrococcus sp. | |
NaCl | loss of 50% activity at 7.5% | Micrococcus sp. | |
Ni2+ | - |
Micrococcus sp. | |
Zn2+ | - |
Micrococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
alpha-naphthyl caprylate | pH 7.5, 30°C | Micrococcus sp. | |
0.08 | - |
alpha-naphthyl butyrate | pH 7.5, 30°C | Micrococcus sp. | |
0.6 | - |
tricaprylin | pH 7.5, 30°C | Micrococcus sp. | |
5 | - |
tributyrin | pH 7.5, 30°C | Micrococcus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion to the culture medium | Micrococcus sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by Mg2+, Ca2+, K+, Na+ | Micrococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44600 | - |
x * 44600, SDS-PAGE | Micrococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus sp. | - |
- |
- |
Micrococcus sp. INIA 528 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from culture supernatant to homogeneity, 296fold | Micrococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
239.5 | - |
purified enzyme | Micrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-naphthyl butyrate + H2O | preferred substrate | Micrococcus sp. | alpha-naphthol + butyrate | - |
? | |
alpha-naphthyl butyrate + H2O | preferred substrate | Micrococcus sp. INIA 528 | alpha-naphthol + butyrate | - |
? | |
alpha-naphthyl caprylate + H2O | 58% of the activity with the alpha-isomer | Micrococcus sp. | alpha-naphthol + caprylate | - |
? | |
alpha-naphthyl caprylate + H2O | 58% of the activity with the alpha-isomer | Micrococcus sp. INIA 528 | alpha-naphthol + caprylate | - |
? | |
alpha-naphthyl laurate + H2O | - |
Micrococcus sp. | alpha-naphthol + laurate | - |
? | |
alpha-naphthyl stearate + H2O | equally active with both isomers | Micrococcus sp. | alpha-naphthol + stearate | - |
? | |
beta-naphthyl butyrate + H2O | 58% of the activity with the alpha-isomer | Micrococcus sp. | beta-naphthol + butyrate | - |
? | |
beta-naphthyl caprylate + H2O | - |
Micrococcus sp. | beta-naphthol + caprylate | - |
? | |
beta-naphthyl laurate + H2O | 62.5% of the activity with the alpha-isomer | Micrococcus sp. | beta-naphthol + laurate | - |
? | |
beta-naphthyl stearate + H2O | equally active with both isomers | Micrococcus sp. | beta-naphthol + stearate | - |
? | |
additional information | the enzyme hydrolyzes alpha-naphthyl esters of fatty acid with chain lengths from C4 to C18 | Micrococcus sp. | ? | - |
? | |
additional information | the enzyme hydrolyzes alpha-naphthyl esters of fatty acid with chain lengths from C4 to C18 | Micrococcus sp. INIA 528 | ? | - |
? | |
tributyrin + H2O | best substrate | Micrococcus sp. | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | best substrate | Micrococcus sp. INIA 528 | dibutyrin + butyrate | - |
? | |
tricaprylin + H2O | - |
Micrococcus sp. | dicaprylin + caprylate | - |
? | |
trilaurin + H2O | - |
Micrococcus sp. | dilaurin + laurate | - |
? | |
trilaurin + H2O | - |
Micrococcus sp. INIA 528 | dilaurin + laurate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44600, SDS-PAGE | Micrococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
tributyrin esterase | - |
Micrococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Micrococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | 80% remaining activity | Micrococcus sp. |
50 | - |
5 min, 50% remaining activity | Micrococcus sp. |
60 | 70 | less than 20% activity after 2 min | Micrococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Micrococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | rapid decrease in activity below pH 6.0 and above pH 8.5 | Micrococcus sp. |