Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | BTID-B, slight activation | Geobacillus thermoleovorans | |
Co2+ | BTID-B, slight activation | Geobacillus thermoleovorans | |
K+ | BTID-B, slight activation | Geobacillus thermoleovorans | |
Mn2+ | BTID-B, slight activation | Geobacillus thermoleovorans | |
Na+ | BTID-B, slight activation | Geobacillus thermoleovorans |
Cloned (Comment) | Organism |
---|---|
BTID-B, DNA sequence determination and analysis, expression in Escherichia coli | Geobacillus thermoleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | BTID-A, 26% inhibition at 1% w/v | Geobacillus thermoleovorans | |
Ag+ | BTID-A | Geobacillus thermoleovorans | |
Ca2+ | BTID-A, slight inhibition | Geobacillus thermoleovorans | |
Co2+ | BTID-A | Geobacillus thermoleovorans | |
Cu2+ | BTID-A, BTID-B | Geobacillus thermoleovorans | |
dithiothreitol | BTID-A, 48% inhibition at 1% w/v | Geobacillus thermoleovorans | |
DMSO | BTID-A, 54% inhibition at 1% w/v | Geobacillus thermoleovorans | |
EDTA | BTID-B, 45% inhibition at 1% w/v, BTID-A, 68% inhibition at 1% w/v | Geobacillus thermoleovorans | |
ethanol | BTID-A, 42% inhibition at 1% w/v | Geobacillus thermoleovorans | |
Fe2+ | BTID-A, BTID-B | Geobacillus thermoleovorans | |
Hg2+ | BTID-A, BTID-B | Geobacillus thermoleovorans | |
K+ | BTID-A | Geobacillus thermoleovorans | |
Mg2+ | BTID-A | Geobacillus thermoleovorans | |
Mn2+ | BTID-A | Geobacillus thermoleovorans | |
Na+ | BTID-A | Geobacillus thermoleovorans | |
PMSF | BTID-A, 92% inhibition at 1% w/v | Geobacillus thermoleovorans | |
SDS | BTID-B, complete inhibition at 1% w/v, no inhibition of BTID-A | Geobacillus thermoleovorans | |
Zn2+ | BTID-A, BTID-B | Geobacillus thermoleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.82 | - |
tricaprylin | BTID-A, pH 7.5, 60°C | Geobacillus thermoleovorans | |
6.24 | - |
tricaprylin | BTID-B, pH 7.5, 60°C | Geobacillus thermoleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18000, BTID-A, SDS-PAGE, 1* 43000, BTID-B, SDS-PAGE | Geobacillus thermoleovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Geobacillus thermoleovorans | enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources | ? | - |
? | |
additional information | Geobacillus thermoleovorans ID-1 | enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources | ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | BTID-B is strongly resistant to organic solvents | Geobacillus thermoleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermoleovorans | - |
2 thermostable lipases A and B, BTID-A and BTID-B | - |
Geobacillus thermoleovorans ID-1 | - |
2 thermostable lipases A and B, BTID-A and BTID-B | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, BTID-A 300fold, recombinant BTID-B 107.5fold | Geobacillus thermoleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
purified BTID-A | Geobacillus thermoleovorans |
292 | - |
purified recombinant BTID-B | Geobacillus thermoleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2,3-trioleoyl-glycerol + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
1,2-dilinoleoyl-3-oleoyl-rac-glycerol + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
1,3-dioleoyl-2-palmitoyl-glycerol + H2O | not BTID-A | Geobacillus thermoleovorans | ? | - |
? | |
1,3-dipalmitoyl-2-oleoyl-glycerol + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
additional information | enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources | Geobacillus thermoleovorans | ? | - |
? | |
additional information | enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources | Geobacillus thermoleovorans ID-1 | ? | - |
? | |
tributyrin + H2O | not BTID-A | Geobacillus thermoleovorans | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | not BTID-A | Geobacillus thermoleovorans ID-1 | dibutyrin + butyrate | - |
? | |
tricaprin + H2O | - |
Geobacillus thermoleovorans | dicaprin + decaprate | - |
? | |
tricaproin + H2O | not BTID-A | Geobacillus thermoleovorans | dicaproin + caproate | - |
? | |
tricaprylin + H2O | best substrate, BTID-A and BTID-B | Geobacillus thermoleovorans | dicaprylin + caprylate | - |
? | |
tricaprylin + H2O | best substrate, BTID-A and BTID-B | Geobacillus thermoleovorans ID-1 | dicaprylin + caprylate | - |
? | |
trilaurin + H2O | - |
Geobacillus thermoleovorans | dilaurin + laurate | - |
? | |
trilaurin + H2O | - |
Geobacillus thermoleovorans ID-1 | dilaurin + laurate | - |
? | |
trimyristin + H2O | - |
Geobacillus thermoleovorans | dimyristin + myristate | - |
? | |
tripalmitin + H2O | - |
Geobacillus thermoleovorans | dipalmitin + palmitate | - |
? | |
tripalmitin + H2O | - |
Geobacillus thermoleovorans ID-1 | dipalmitin + palmitate | - |
? | |
tristearin + H2O | - |
Geobacillus thermoleovorans | distearin + stearate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18000, BTID-A, SDS-PAGE, 1* 43000, BTID-B, SDS-PAGE | Geobacillus thermoleovorans |
Synonyms | Comment | Organism |
---|---|---|
BTID | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
BTID-B | Geobacillus thermoleovorans |
60 | 65 | BTID-A | Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
BTID-B is stable for 2 h, BTID-A shows over 75% remaining activity after 30 min | Geobacillus thermoleovorans |
70 | - |
BTID-B, 30 min, 50% remaining activity | Geobacillus thermoleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | BTID-B | Geobacillus thermoleovorans |
9 | - |
BTID-A | Geobacillus thermoleovorans |