Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystal structure at 1.3 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D93N | 4% of wild-type kcat | Escherichia coli |
H20N | no activity | Escherichia coli |
H20Q | no activity | Escherichia coli |
K103Q | 54% of wild-type kcat | Escherichia coli |
K103R | 68% of wild-type kcat | Escherichia coli |
K103S | 28% of wild-type kcat | Escherichia coli |
K105Q | 20% of wild-type kcat | Escherichia coli |
K105R | 26% of wild-type kcat | Escherichia coli |
K105S | 16% of wild-type kcat | Escherichia coli |
K113Q | 98% of wild-type kacat | Escherichia coli |
M67E | 0.5% of wild-type kcat | Escherichia coli |
N10D | 0.05% of wild-type kcat | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | 0.5 mM, 90% inactivation after 10 min, activity can be recovered to 41% of initial activity by treatment wit 200 mM hydroxylamine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
Oregon Green-methionine-tRNA | 25°C, pH 7.5 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-substituted aminoacyl-tRNA + H2O | Escherichia coli | - |
N-substituted amino acid + tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Pth | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-substituted aminoacyl-tRNA + H2O | - |
Escherichia coli | N-substituted amino acid + tRNA | - |
? | |
Oregon Green-methionine-tRNA + H2O | - |
Escherichia coli | Oregon Green-methionine + tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PTH | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.3 | - |
Oregon Green-methionine-tRNA | 25°C, pH 7.5 | Escherichia coli |