Cloned (Comment) | Organism |
---|---|
gene estB, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha | Sphingobium sp. SM42 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium sp. SM42 | A0A0C5DFF8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography | Sphingobium sp. SM42 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Sphingobium sp. SM42 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Sphingobium sp. SM42 | 4-nitrophenol + butyrate | - |
? | |
cefazolin + H2O | 67% conversion | Sphingobium sp. SM42 | ? | - |
? | |
cefoperazone + H2O | 83% conversion | Sphingobium sp. SM42 | (2R)-2-[(4-ethyl-2,3-dioxopiperazinyl)carbonylamino]-2-(4-hydroxyphenyl)acetic acid + 1-methyl-1H-tetrazole-5-thiol + ? | - |
? | |
cefotaxime + H2O | 16% conversion | Sphingobium sp. SM42 | ? | - |
? | |
ceftriaxone + H2O | 18% conversion | Sphingobium sp. SM42 | ? | - |
? | |
dibutyl phthalate + H2O | - |
Sphingobium sp. SM42 | ? | - |
? | |
additional information | the enzyme EstB is active towards dibutyl phthalate, and can cleave some small aromatic ring side chains from cephalosporin derivatives. No activity against cephalosporin C and cefixime. EstB catalyzed the cleavage of the C-S bond found in cephalosporin derivatives to release the corresponding free aromatic ring side chains. EstB is active towards short-chain nitrophenyl esters (i.e. 4-nitrophenyl acetate (C2) and 4-nitrophenyl butyrate (C4)) and towards tributyrin. Substrate specificity of the Sphingobium sp. SM42 esteraseB (EstB) towards cephalosporin antibiotics | Sphingobium sp. SM42 | ? | - |
? | |
tributyrin + H2O | - |
Sphingobium sp. SM42 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, about, sequence calculation | Sphingobium sp. SM42 |
Synonyms | Comment | Organism |
---|---|---|
de-arenethiolase | - |
Sphingobium sp. SM42 |
EstB | - |
Sphingobium sp. SM42 |
esterase B | - |
Sphingobium sp. SM42 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sphingobium sp. SM42 | sequence calculation | - |
5.3 |
General Information | Comment | Organism |
---|---|---|
physiological function | esterase B from Sphingobium sp. SM42 exhibits de-arenethiolase activity against cephalosporin antibiotics. It is highly active against the third and first generation cephalosporins, cefoperazone and cefazolin and demonstrates a mild activity towards the third generation drugs, ceftriaxone and cefotaxime. The enzyme is one of two esterases whose genes have been isolated from the dibutyl phthalate (DBP)-degrading soil bacterium Sphingobium sp. SM42 | Sphingobium sp. SM42 |