Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 | Streptococcus equi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | complete inhibition at 0.01 mM | Streptococcus equi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
Acetylsalicylic acid | in 2.0 mM Tris-HCl, pH 7.6, at 25°C | Streptococcus equi | |
29 | - |
tripropionin | in 2.0 mM Tris-HCl, pH 7.6, at 25°C | Streptococcus equi | |
56 | - |
ethyl acetate | in 2.0 mM Tris-HCl, pH 7.6, at 25°C | Streptococcus equi | |
68 | - |
vinyl propionate | in 2.0 mM Tris-HCl, pH 7.6, at 25°C | Streptococcus equi | |
203 | - |
Triacetin | in 2.0 mM Tris-HCl, pH 7.6, at 25°C | Streptococcus equi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus equi | B5THG6 | subspecies Streptococcus equi equi | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and DEAE-Sepharose column chromatography | Streptococcus equi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
using tributyrin as substrate, at 25°C | Streptococcus equi |
24 | - |
using vinyl butyrate as substrate, at 25°C | Streptococcus equi |
44 | - |
using acetylsalicyclic acid as substrate, at 25°C | Streptococcus equi |
68 | - |
using ethyl acetate as substrate, at 25°C | Streptococcus equi |
149 | - |
using tripropionin as substrate, at 25°C | Streptococcus equi |
513 | - |
using triacetin as substrate, at 25°C | Streptococcus equi |
815 | - |
using vinyl propionate as substrate, at 25°C | Streptococcus equi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Streptococcus equi | 4-nitrophenol + acetate | - |
? | |
acetylsalicylic acid + H2O | - |
Streptococcus equi | salicylic acid + acetate | - |
? | |
ethyl acetate + H2O | - |
Streptococcus equi | ethanol + acetate | - |
? | |
additional information | does not hydrolyze ethyl butyrate, trioctanoin, vinyl laurate, acetylcholine, and triglycerides | Streptococcus equi | ? | - |
? | |
triacetin + H2O | - |
Streptococcus equi | acetic acid + glycerol | - |
? | |
tributyrin + H2O | - |
Streptococcus equi | butanoate + glycerol | - |
? | |
tripropionin + H2O | - |
Streptococcus equi | propionic acid + glycerol | - |
? | |
vinyl butyrate + H2O | - |
Streptococcus equi | vinyl alcohol + butyrate | - |
? | |
vinyl propionate + H2O | - |
Streptococcus equi | vinyl alcohol + propionate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SeE | - |
Streptococcus equi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptococcus equi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | the reaction rates dramatically slow down with time at 50 and 60°C | Streptococcus equi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptococcus equi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | the activities of SeE after 1 h incubation at pH 4 and 5 are less than 20% of the activity of SeE after 1 h incubation at pH 7, whereas only a slight decrease is observed after incubation at pH 8 and 9 | Streptococcus equi |