Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
the gene that has a deletion of about 60 residues at the N terminus(SSODELTAN) is expressed in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
M211S/R215L | about 50% decrease both in kcat- and in KM-value | Alicyclobacillus acidocaldarius |
additional information | truncated version of wild-type and mutant M211S/R215L lacking N-terminal 36 amino acids. Both truncated enzymes show reduction of temperature optimum to 50°C but pH-optimum of 7.1 similar to wild-type | Alicyclobacillus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
4-nitrophenyl hexanoate | pH 7.1, 70°C, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | |
50 | - |
p-nitrophenyl hexanoate | pH 7.1, 70°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus acidocaldarius | - |
isoform EST2 | - |
Saccharolobus solfataricus | - |
isoform EST2 | - |
Saccharolobus solfataricus | Q97VU2 | - |
- |
Saccharolobus solfataricus P2 | Q97VU2 | - |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus(SSODELTAN) | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.3 | - |
pH 7.1, 70°C | Saccharolobus solfataricus |
4.3 | - |
pH 7.1, 70°C, substrate: 4-nitrophenyl hexanoate, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl dodecanoate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | 4-nitrophenol + dodecanoate | - |
? | |
4-nitrophenyl dodecanoate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus P2 | 4-nitrophenol + dodecanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus P2 | 4-nitrophenol + hexanoate | - |
? | |
glyceryl tributyrate + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
glyceryl tributyrate + H2O | - |
Alicyclobacillus acidocaldarius | ? | - |
? | |
glyceryl tributyrate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | ? | - |
? | |
glyceryl tributyrate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus P2 | ? | - |
? | |
glyceryl tridecanoate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | ? | - |
? | |
glyceryl tridecanoate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus P2 | ? | - |
? | |
glyceryl trihexanoate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | ? | - |
? | |
glyceryl trioctanoate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | ? | - |
? | |
glyceryl trioleate + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
glyceryl trioleate + H2O | - |
Alicyclobacillus acidocaldarius | ? | - |
? | |
glyceryl trioleate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | ? | - |
? | |
glyceryl trioleate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus P2 | ? | - |
? | |
p-nitrophenyl hexanoate + H2O | - |
Saccharolobus solfataricus | p-nitrophenol + hexanoate | - |
? | |
p-nitrophenyl hexanoate + H2O | - |
Alicyclobacillus acidocaldarius | p-nitrophenol + hexanoate | - |
? | |
S-methyl thiobutanoate + H2O | activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | methanol + thiobutanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EST2 | - |
Saccharolobus solfataricus |
esterase 2 | - |
Saccharolobus solfataricus |
SSO2517 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
truncated wild-type and M211S/R215L mutant enzyme lacking N-terminal 36 amino acids | Alicyclobacillus acidocaldarius |
70 | - |
- |
Saccharolobus solfataricus |
70 | - |
wild-type | Alicyclobacillus acidocaldarius |
70 | - |
substrate: 4-nitrophenyl hexanoate, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 75 | 60°C: 55% of maximal activity, 75°C: about 35% of maximal activity, substrate: 4-nitrophenyl hexanoate, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable for more than 10 h | Saccharolobus solfataricus |
70 | - |
wild-type, 3 h, no loss of activity, wild-type lacking N-terminal 36 amino acids, 70% residual activity, mutant M211S/R215L lacking N-terminal 36 amino acids, 30% residual activity | Alicyclobacillus acidocaldarius |
70 | - |
stable for more than 10 h, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.47 | - |
p-nitrophenyl hexanoate | pH 7.1, 70°C | Saccharolobus solfataricus | |
2.47 | - |
4-nitrophenyl hexanoate | pH 7.1, 70°C, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus | |
5 | 15 | p-nitrophenyl hexanoate | pH 7.1, 50°C, wild-type lacking N-terminal 36 amino acids | Alicyclobacillus acidocaldarius | |
508 | - |
p-nitrophenyl hexanoate | pH 7.1, 50°C, mutant M211S/R215L lacking N-terminal 36 amino acids | Alicyclobacillus acidocaldarius | |
2300 | - |
p-nitrophenyl hexanoate | pH 7.1, 50°C, mutant M211S/R215L | Alicyclobacillus acidocaldarius | |
4320 | - |
p-nitrophenyl hexanoate | pH 7.1, 50°C, wild-type | Alicyclobacillus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
at 70°C | Saccharolobus solfataricus |
7.1 | - |
wild-type and N-terminal deletion mutant | Alicyclobacillus acidocaldarius |
7.1 | - |
substrate: 4-nitrophenyl hexanoate, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | pH 4.0: about 40% of maximal activity, pH 10.0: aboutv 40% of maximal activity, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
49.4 | - |
4-nitrophenyl hexanoate | pH 7.1, 70°C, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus | Saccharolobus solfataricus |